Sequence analysis of enterovirus 71 3′UTR and construction of a recombinant cDNA infectious clone
10.3760/cma.j.issn.1003-9279.2019.01.019
- VernacularTitle: 肠道病毒71型3′非编码区序列分析及重组病毒的构建
- Author:
Zhen DONG
1
;
Lihong WANG
1
;
Ziwei LIU
1
;
Rui CHEN
1
;
Zexin TAO
2
;
Li ZHAO
1
;
Zhiyu WANG
1
;
Hongling WEN
1
Author Information
1. Department of Microbiological Laboratory Technology, School of Public Health, Shandong University, Key Laboratory for the Prevention and Control of Infectious Diseases (Key Laboratory of the "13th Five-Year" , Shandong Colleges and Universities), Jinan 250012, China
2. Shandong Provincial Center for Disease Control and Prevention, Shandong Provincial Key Laboratory for Infectious Disease Control and Prevention, Jinan 250012, China
- Publication Type:Journal Article
- Keywords:
Enterovirus 71;
Untranslated region;
Recombinant virus
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):83-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To sequence the 3′UTR of enterovirus 71 strains, investigate its foundation and impact in virulence by constructing a 3′UTR-replaced recombinant cDNA infectious clone.
Methods:Viral RNA of EV-A71 isolated viruses were extracted, and the nucleotide analysis was performed after sequencing. The 3′UTR of a full-length infectious clone of SDLY107 strain was replaced by its corresponding part of SDLY1 strain, and then the recombinant virus was constructed and identified.
Results:The nine isolated strains were classified into sub-genotype C4a of enterovirus (EV)-A71 by analysis, and nucleotide sequence homology for 3′UTR were 94%-100%. 3′UTR of EV-A71 strains may be associated with its pathogenicity. Identification of the rescued virus by sequencing and indirect immunofluorescence confirmed the successful construction of infectious recombinant virus.
Conclusions:Sequence analysis indicated that the 3′UTR may be involved in the pathogenicity of EV-A71. The recombinant virus SDLY107(1-3′UTR) was rescued successfully. Our study may provide evidence for further research on the influence of 3′UTR on the virulence of enterovirus 71.
