IL-32 expression in peripheral blood mononuclear cells of chronic hepatitis B virus-infected patients with PEG IFN-α-2a treated and its significance
10.3760/cma.j.issn.1003-9279.2019.01.018
- VernacularTitle: 聚乙二醇干扰素α-2a治疗慢性乙型肝炎患者外周血IL-32的表达水平及意义
- Author:
Yongping LIU
1
;
Huihua GU
;
Jing XU
;
Yan ZHENG
;
Wei HUANG
;
Chun ZHAO
;
Yan LIU
Author Information
1. Department of Hepatology, Xixi Hospital of Hangzhou, Hangzhou 310023, China
- Publication Type:Journal Article
- Keywords:
Interleukin-32;
Chronic Hepatitis B;
PEG IFN-α
-2a;
Liver;
Inflammation
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):79-82
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression level of IL-32 in peripheral blood mononuclear cells (PBMCs) and its correlation with serum biochemical indices of liver function test and HBV DNA load in chronic hepatitis B (CHB) patients with PEG IFN-α-2a treated.
Methods:Thirty CHB patients with PEG IFN-α-2a treated (CHB group) and thirty normal health donors (health group) were enrolled in the study. Total RNA in PBMCs was extracted by using TRIzol. Than IL-32 mRNA level was assayed by using Real-time PCR. The correlation between IL-32 and ALT, AST, TBIL, HBV DNA load was analyzed using pearson′s correlation analysis, respectively.
Results:IL-32 expression level in CHB group was significantly lower than that of health group. Moreover, the difference between them was statistically significant (P=0.000). IL-32 level was positively correlated with serum ALT and AST, respectively (P=0.035, P=0.032), but was not correlated with ALB and HBV DNA load (P=0.856, P=0.580).
Conclusions:IL-32 expression level was decreased in CHB patients with PEG IFN-α-2a Treated and was associated with the severity of inflammation.
