Expression and analysis of histo-blood group antigens binding pattern of GⅡ.2 norovirus P protein
10.3760/cma.j.issn.1003-9279.2019.01.012
- VernacularTitle: GⅡ.2型诺如病毒P蛋白的表达及组织血型抗原结合模式分析
- Author:
Jiao SONG
1
;
Hanbo LI
1
;
Xin CONG
2
;
Miao JIN
2
;
Dandi LI
1
;
Guisen ZHENG
1
;
Zhaojun DUAN
2
Author Information
1. Gansu University of Traditional Chinese Medicine, School of Public Health, Lanzhou 730000, China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Norovirus;
Outbreaks;
P protein;
Histo-blood group antigens
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):54-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective:During the 2016 winter season, GII.2 norovirus(NoV) suddenly emerged in China. To elucidate its mechanism of epidemic, this study focused on characteristics of binding between the P protein of capsid and histo-blood group antigens (HBGAs).
Methods:The research object was GⅡ.2 ZTX strain which had an outbreaks by the end of 2016 in Beijing. Recombinant prokaryotic expression plasmid was constructed, and the expression of virus P protein was determined and purified. The P protein characteristics of binding to HBGAs was studied through saliva and oligosaccharide binding experiments.
Results:Soluble P protein was successfully obtained, and combined with type A, B, AB saliva.
Conclusions:The result illuminate the combination with new outbreaks of NoV and salivary types, which provided a basis for its pathogenic mechanism and prevention and control measures.
