Analysis of epidemic characteristics of rotavirus diarrhea in children under 5 years in Lanzhou, 2017
10.3760/cma.j.issn.1003-9279.2019.01.011
- VernacularTitle: 2017年兰州市5岁以下儿童轮状病毒腹泻流行特点分析
- Author:
Fang WANG
1
;
Qing ZHANG
2
;
Dandi LI
2
;
Yuning LI
1
Author Information
1. Department of Pediatrics, First Hospital of Lanzhou University, Lanzhou 730000, China
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Diseases Control and Prevention, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Rotavirus A;
Children;
Genotype
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):49-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the infection status and pathogenic characteristics of rotavirus in group A of children under 5 years of age in Lanzhou city in 2017, and provide scientific data for prevention and control of rotavirus infection.
Methods:A total of 219 stool samples from children with diarrhea under the age of 5 years in the Department of Pediatrics, First Hospital of Lanzhou University from January to December in 2017 were collected. A group of human rotavirus positive samples were detected by ELISA, and G/P typing was detected by using reverse transcription-polymerase chain reaction (RT-PCR)) combined with sequencing. Some of the VP7 gene fragments were amplified, sequenced and analyzed for phylogenetic characteristics.
Results:A total of 113 rotavirus in group A positive samples were detected, with a total positive rate of 51.60%. The main target of infection was children 0 to 17 months, and the peak of the epidemic was mainly in November. G/P genotyping was performed: G9 was predominant G genotype (90.27%), followed by G2 (7.08%). P[8] was predominant P genotype (91.15%), followed by P [4] (7.96%). In 2017, the group A human rotavirus epidemic strain was mainly G9P[8](88.50%). Sequence analysis and phylogenetic analysis of VP7 gene fragments showed that the dominant genotypes G9 had higher homology with isotype reference strains in other regions of China.
Conclusions:Children 0 to 17 months old in Lanzhou city are at high risk of group A human rotavirus infection. G9P[8] is a dominant genotype.
