Characteristics of Coxsackievirus A16 VP1 gene in Jinan, 2017
10.3760/cma.j.issn.1003-9279.2019.01.010
- VernacularTitle: 2017年济南市柯萨奇病毒A16型VP1基因特征分析
- Author:
Huailong ZHAO
1
;
Hongtao SUI
2
;
Guoliang YANG
1
;
Hengyun GUAN
1
;
Chunrong WANG
1
;
Lanzheng LIU
1
Author Information
1. Jinan Municipal Center for Disease Control and Prevention, Jinan 250021, China
2. Institute of Pathogen Biology Chinese Academy of Medical Sciences, Beijing 100730, China
- Publication Type:Journal Article
- Keywords:
Hand, foot and mouth disease;
Coxsaekievirus A16;
Phylogenetic tree
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):44-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the VP1 gene characteristics of Coxsackievirus A16 (CV-A16) isolated in(hand, foot and mouth disease, HFMD) patients of Jinan 2017.
Methods:The samples collected from patients with HFMD in Jinan city in 2017 were analyzed and some of the CV-A16 nucleic acid positive samples were choosen with simple random sampling method and pretreated to inoculate RD cells. The whole VP1 gene sequences of CV-A16 stains which had obvious cytopathic effect in RD cells were amplified and sequenced. MEGA5.2 software was used to constructed phylogenetic tree and Lasergene software was used to analysis the homology consistency of nucleotide and amino acid of VP1 gene.
Results:All the 10 CV-A16 strains isolated from Jinan were located 3 clusters belonged to B1b genotypes, with a nucleotide similarity of 94.9%~100% and deduced amino acid similarity of 99.3%~100%. The Jinan CV-A16 strains were nearest related with Shanghai strains KX871333(nucleotide similarity: 99.7%), Henan strains KM260134(99.0%), Jiangsu strains KP751580、KR138327(98.7%) and Guangdong strains MH004016(98.5%). Compared with some reference strains from our country, amino acid mutation were identified at positions 14, 59, and 145 in VP1 proteins of Jinan CV-A16 strains.
Conclusions:CV-A16 strains B1b genotypes were the strains prevailed in Jinan city, and 3 amino acid substitutions in VP1 proteins were happened in CV-A16 strains isolated from Jinan.
