Molecular etiological characteristics of the gastroenteritis outbreak associated with norovirus infection in Guangyuan city, Sichuan province
10.3760/cma.j.issn.1003-9279.2019.01.009
- VernacularTitle: 四川省广元市胃肠炎暴发中诺如病毒的分子病原学特征研究
- Author:
Xueqin HOU
1
;
Min REN
2
;
Li XIONG
1
;
Jun ZHU
2
;
Ranran CAO
1
;
Xiaozhen MA
2
Author Information
1. Guangyuan Center for Disease Control and Prevention, Guangyuan 628000, China
2. Sichuan Center for Disease Control and Prevention, Chengdu 610041, China
- Publication Type:Journal Article
- Keywords:
Norovirus;
Gastroenteritis;
Outbreak;
Phylogenetic analysis
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):39-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the genotype and genetic characteristics of the pathogens associated with the epidemic outbreak of acute gastroenteritis(AGE) in Guangyuan city, Sichuan province.
Methods:Eighteen stool samples and 15 anal swab samples from 4 AGE outbreaks were collected from Feb 2017 to May 2017. Norovirus (NoV) nucleic acid was detected by Real-time PCR method , and the positive samples were amplified by conventional reverse transcription-polymerase chain reaction (RT-PCR) and nucleotide sequencing.
Results:Four AGE outbreaks were all caused by NoV. And 20 (60.6%) samples were positive for NoV GⅡ. Gene sequence comparison and phylogenetic analysis showed that 4 AGE outbreaks were all caused by GⅡ.P16/GⅡ.2 NoV. All the strains of GⅡ.P16/GⅡ.2 NoV shared high homology in nucleotides. One of the outbreaks was caused by GⅡ.P16/GⅡ.2 and GⅡ.P7/GⅡ.14 NoV co-infection.
Conclusions:The 4 outbreaks of AGE in Guangyuan city, Sichuan province were major caused by GⅡ.P16/GⅡ.2 NoV, meanwhile GⅡ.P16/GⅡ.2 and GⅡ.P7/GⅡ.14 NoV co-infection existed.
