A autophagy induced by non-stuctural protein 2B of human rhinovirus
10.3760/cma.j.issn.1003-9279.2019.01.005
- VernacularTitle: 鼻病毒非结构蛋白2B引起细胞自噬产生的研究
- Author:
Juan SONG
1
,
2
;
Xiaohui ZOU
;
Xiaonuan LUO
;
Qinqin SONG
;
Bingtian SHI
;
Dong XIA
;
Mi LIU
;
Zhiqiang XIA
;
Zhuozhuang LU
;
Jun HAN
Author Information
1. State Key Laboratory of Infectious Disease Prevention and Control
2. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China
- Publication Type:Journal Article
- Keywords:
MiniSOG;
2B protein;
Autophagy
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):21-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the intracellular location and autophagosome production of rhinovirus 16 2B protein using miniSOG labeling technique.
Methods:2B was fused with miniSOG and flag tags to construct pcDNA3.1-2B-miniSOG-flag plasmid, which was used to transfect HEK293 cells, LC3 protein was detected by western blot. The transfected cells were fixed, stained with DAB through the photooxidation activity of miniSOG, and used to prepare ultrathin sections. Localization of 2B-miniSOG protein in cells and ultrastructural changes of cells were observed under electron microscope.
Results:2B-miniSOG protein glows green under a fluorescence microscopy. Green flourescence coold be observed in the cells expressing 2B-miniSOG protein.LC-II protein increased in the cells transfected with pcDNA3.1-2B-miniSOG-flag. Under electron microscopy it was observed that 2B-miniSOG protein was located in the mitochondria, and a large number of vesicular structures appeared in the cytoplasm. Both autophagosomes and autophagic lysosomes can be observed.
Conclusions:Non-structural protein 2B of HRV16 can induce autophagy.
