Pathogenic composition of hand, foot and mouth disease and enterovirus-A71 and coxsackievirus-A16 genetic characteristics analysis in Ningxia, 2016-2017
10.3760/cma.j.issn.1003-9279.2019.01.003
- VernacularTitle: 2016—2017年宁夏手足口病的病原构成及流行的EV-A71和CV-A16基因特征分析
- Author:
Jie LI
1
;
Jiangtao MA
2
;
Li LIU
3
;
Yong ZHANG
1
;
Tianjiao JI
1
;
Wenbo XU
1
;
Dongmei YAN
1
;
Guizhen WU
1
Author Information
1. NHC Key Laboratory of Medical Virology and Viral Disease, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China
2. Ningxia Center for Disease Control and Prevention, Yinchuan 750004, China
3. Haidian Center for Disease Control and Prevention, Beijing 100089, China
- Publication Type:Journal Article
- Keywords:
Hand, foot and mouth disease;
Pathogenic composition;
Public health;
Molecular epidemiology
- From:
Chinese Journal of Experimental and Clinical Virology
2019;33(1):10-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the pathogenic composition of hand, foot and mouth disease (HFMD) in Ningxia Hui Autonomous Region (Ningxia) from 2016 to 2017, and analyze the genetic characteristics of the main pathogens enterovirus (EV)-A71 and coxsackievirus (CV)-A16.
Methods:Analysis of the result of nucleic acid testing of HFMD in Ningxia from 2016 to 2017 to determine the pathogenic composition of HFMD. The complete VP1 coding region was amplified by RT-PCR and the gene sequence was determined for the enterovirus strains sent to the National HFMD Network Monitoring Laboratory in Ningxia from 2016 to 2017. BLAST analysis confirmed the serotype of the strain, and the phylogenetic tree was constructed respectively by selecting EV-A71 and CV-A16 isolates.
Results:The leading pathogens of HFMD in Ningxia of 2016 and 2017 were other EV (397, 43.72%) and EV-A71 (918, 56.18%) respectively, and the dominant pathogens in different months may differ. The pathogenic composition causing HFMD in the past two years has changed from CV-A16 and other EV to EV-A71 and other EV. The isolated EV-A71 strains were C4a evolutionary branch and the isolated CV-A16 strains were B1b evolutionary branch.
Conclusions:Compared to 2016, in 2017 EV-A71, CV-A16 and other EV changed dynamically. Dynamic monitoring of EV-A71 in Ningxia is of great significance to guide the strategy of using EV-A71 vaccine, concentrating medical resources to strengthen the treatment and reduce the mortality rate of severe HFMD cases.
