Repair effect of adipose-derived mesenchymal stem cells on lung injury in rats exposed to silica
10.3760/cma.j.issn.1001-9391.2019.01.004
- VernacularTitle: 脂肪间充质干细胞对染矽尘大鼠肺损伤修复的研究
- Author:
Shangya CHEN
1
;
Ru HAN
1
;
Enguo ZHANG
1
,
2
;
Ye YANG
1
,
2
;
Qiang JIA
1
;
Linlin SAI
1
;
Cunxiang BO
1
;
Yu ZHANG
1
;
Zhongjun DU
1
,
2
;
Hua SHAO
1
,
2
Author Information
1. Shandong Academy of Occupational Health and Occupational Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China
2. School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, Jinan University, Jinan 250062, China
- Publication Type:Journal Article
- Keywords:
Adipose-derived mesenchymal stem cells;
Silicosis;
Rats;
Fibrosis;
Apoptosis
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(1):20-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the repairing effect of adipose mesenchymal stem cells (ADSCs) on lung injury induced by silica in rats.
Methods:Primary ADSCs-GFP was obtained from rats. ADSCs-GFP was injected into tail vein of silicosis model rats. The expression of green fluorescence in lungs was observed regularly to determine the homing ability of ADSCs. Primary ADSCs of rats were obtained and randomly divided into control group, exposure group, vehicle group and ADSCs group. Silicosis rat model was established by non-exposed tracheal drip method. 24 hours after silica exposure, rats in ADSCs group were injected with ADSCs of 1×106/kg body weight through tail vein, and the pathological changes of lung tissue were observed and evaluated 28 days after intervention. To explore the early intervention mechanism of ADSCs on pulmonary fibrosis in silicosis model rats, apoptosis-related proteins were detected by immunohistochemistry.
Results:28 days after exposure to silica, rats in the exposure group showed obvious pulmonary fibrosis. Compared with exposure group and vehicle group, ADSCs group showed less pulmonary inflammation, less silica nodules and less collagen deposition area. Immunohistochemical results showed that the expression of Caspase-3 and cytochrome C protein decreased and Bcl-2 protein increased after ADSCs transplantation.
Conclusion:ADSCs infusion has an obvious intervention effect on postponing early silicosis fibrosis in rats exposed to silica, and its mechanism is related to the regulation of apoptotic process.
