Mechanism of lncRNA CTD-3252C9.4 on invasion and migration of pancreatic cancer Panc-1 cells in vitro

VernacularTitle:LncRNA CTD-3252C9.4抑制人胰腺癌Panc-1细胞体外侵袭迁移的机制
Author: Xin YIN ¹ ; Wanyue SHI ; Yi PAN ; Liang JIN
Author Information

1. 中国药科大学生命科学与技术学院
Publication Type:Journal Article
Keywords: lncRNA; bone morphogenetix protein 7; pancreatic cancer; invasion and metastasis; epithelial-mesenchymal transition
From: Journal of China Pharmaceutical University 2019;50(2):230-237
CountryChina
Language:Chinese
Abstract: The aim of this study is to investigate the inhibitory effect of long non-coding RNA(lncRNA)CTD-3252C9. 4 on migration and invasion of human pancreatic cancer cell Panc-1 in vitro and its mechanism. Panc-1 cells were stimulated by epidermal growth factor(EGF)in three-dimensional semi-solid system of cultured pancreatic cancer spheres. RT-qPCR was used to detected the transfection efficiency of lncRNA CTD-3252C9. 4. The effects of lncRNA CTD-3252C9. 4 and bone morphogenetix protein 7(BMP7)on the invasion and migration of Panc-1 cells were detected by scratch healing method and Transwell chamber method. The changes of target gene BMP7 and epithelial-mesenchymal transition(EMT)related proteins were verified by Western blot. EGF could significantly inhibit the expression of lncRNA CTD-3252C9. 4 in Panc-1 cells. The lncRNA can affect cells invasion and migration by inhibiting the transcription of the oncogene BMP7, then inhibit the process of EMT of tumors.