Paeoniflorin inhibits programmed cell death-1-ligand 1 expression in HepG2 cells by regulating JAK/STAT3 signal pathway
10.11665/j.issn.1000-5048.20190213
- VernacularTitle:芍药苷调控JAK/STAT3通路干预HepG2细胞PD-L1表达的研究
- Author:
Nanyan WAN
1
;
Cuihua JIANG
;
Meng GAO
;
Jian ZHANG
;
Zhiqi YIN
;
Ke PAN
Author Information
1. 中国药科大学中药学院天然药物化学系
- Publication Type:Journal Article
- Keywords:
paeoniflorin;
PD-L1;
tumor immune escape;
immune checkpoint
- From:
Journal of China Pharmaceutical University
2019;50(2):213-221
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the effect and its mechanism of paeoniflorin on PD-L1, a PD-L1 high expression cell model was established in interferon gamma(IFN-γ)-induced HepG2 cells. The cytotoxicity of paeoniflorin was detected by MTT assay. Flow cytometry, ELISA and RT-PCR were performed to detect protein and mRNA levels of PD-L1 regulated by paeoniflorin. In HepG2 cells and Jurkat T cell co-culture system, the expression of IL-2 was detected by ELISA. Besides, T cell proliferation was evaluated by CCK-8 method, and the protein expression levels of PD-L1, JAK and STAT3 after drug treatment were determined by Western blot. These results indicated that paeoniflorin could significantly down-regulate the levels of PD-L1 protein and mRNA. In addition, it increased the number of T cells and the concentration of IL-2 in the co-culture system. Furthermore, paeoniflorin could significantly inhibit the protein expression of JAK and STAT3. Au the above experimental data indicated that paeoniflorin could down-regulate the expression of PD-L1, and its mechanism might be related to the JAK/STAT3 pathway.