Determination of the content and related substances of neomycin sulfate by HPLC combined with pulsed amperometric detection
10.11665/j.issn.1000-5048.20190210
- VernacularTitle:HPLC-PAD法测定硫酸新霉素含量及其有关物质
- Author:
Qian ZHANG
1
;
Minwen HUANG
;
Wang FANG
;
Mei ZHANG
;
Taijun HANG
;
Yaozuo YUAN
Author Information
1. 江苏省食品药品监督检验研究院；中国药科大学药物分析教研室
- Publication Type:Journal Article
- Keywords:
high performance liquid chromatography combined with pulsed amperometric detection(HPLC-PAD);
neomycin sulfate;
content;
related substance
- From:
Journal of China Pharmaceutical University
2019;50(2):193-199
- CountryChina
- Language:Chinese
-
Abstract:
A novel method was developed for the content assay and related substances determination of neomycin sulfate by high performance liquid chromatography combined with pulsed amperometric detection(HPLC-PAD). The HPLC was performed on Thermo AcclaimTMAmG C18(4. 6 mm×150 mm, 3 μm). The mobile phase consisted of aqueous solution with 2% trifluoroacetic acid containing 0. 01% pentafluoropropionic acid and 0. 6%NaOH. The pulsed amperometric detector was operated with aquadruple-potential waveform for the detection. Neomycin B, Neomycin C and thirteen related substances were adequately separated by the established HPLC conditions. The limits of detection(LOD)and quantification(LOQ)of neomycin B and neomycin C were both 1. 75 ng and 3. 5 ng, respectively. Good linearities of neomycin B and neomycin C were found in their respective ranges which their correlation coefficients were greater than 0. 998 5. The established method is characterized by high specificity, sensitivity and wide range of linearity which has a good application prospect and provides the basis for improving the standard and quality control of neomycin sulfate.
