The effect of concentration ratio on tumor cell adhesion in shear flow
10.3871/j.1004-7220.2015.05.392
- VernacularTitle:细胞浓度比对剪切流中肿瘤细胞黏附的影响
- Author:
Yi FU
1
;
Yang WANG
2
;
Fan BAI
1
;
Jie WU
1
;
Ren SUN
1
;
Zu-rong DING
1
;
Cheng DONG
1
,
3
Author Information
1. MOE Key Laboratory of Hydrodynamics, School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University
2. Department of Urology, the Fifth People's Hospital of Shanghai, Fudan University School of Medicine
3. Department of Biomedical Engineering, the Pennsylvania State University
- Publication Type:Journal Article
- Keywords:
Concentration ratio;
Endothelial cells (ECs);
Polymorphonuclear neutrophils(PMNs);
Tumor cells (TCs);
Shear rate;
Cell adhesion
- From:
Journal of Medical Biomechanics
2015;30(5):E392-E396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the different effects from different concentration ratios of polymorphonuclear neutrophil (PMN) to tumor cell (TC) on the process of tumor cell adhesion to endothelial cell (EC) in shear flow. Methods PMNs and TCs with different concentration ratios (PMN-TC ratio) were added into the parallel plate flow chamber, and changes in the numbers of transient and accumulative adhered TCs on ECs at different shear rates (50 s-1，100 s-1，200 s-1) were analyzed. Results The transient and accumulative adhesion of TCs on ECs at PMN-TC ratio of 3︰1 significantly increased as compared to that at PMN-TC ratio of 1︰1, especially under high shear flow condition (100 s-1 and 200 s-1). Moreover, in the 5 minute-observation period, the effect of PMN-TC ratio on TC adhered to ECs occurred earlier when the shear rate increased. Conclusions The increase of PMN-TC concentration ratio can promote TC adhesion to ECs in shear flow, and the research findings provide significant references for studying TC metastasis in blood vessels and the target therapy of tumors.
