Shear stress modulates the proliferation of vascular smooth muscle cells via receptor for activated C kinase 1
- VernacularTitle:活化激酶C受体1在切应力调控血管平滑肌细胞增殖中的作用
- Author:
Yan SHEN
1
;
Lu WANG
1
;
Yue HAN
1
;
Yu-chen YANG
1
;
Bao-rong SHEN
1
;
Ying-xin QI
1
;
Zong-lai JIANG
1
Author Information
1. Institute of Mechanobiology and Medical Engineering, School of Life Sciences and and Biotechnology, Shanghai Jiaotong Universit
- Publication Type:Journal Article
- Keywords:
Shear stress;
Endothelial cells (ECs);
Vascular smooth muscle cells (VSMCs);
Receptor for activated C kinase 1 (RACK1);
Cell proliferation
- From:
Journal of Medical Biomechanics
2014;29(6):E491-E497
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of receptor for activated C kinase 1 (RACK1) in vascular smooth muscle cells (VSMCs) proliferation modulated by co-cultured endothelial cells (ECs) and shear stress. Methods Using EC/VSMC co-cultured parallel plate flow chamber system, two levels of shear stress, i.e. low shear stress (LowSS, 0.5 Pa) and normal shear stress (NSS, 1.5 Pa), were applied for 12 h. BrdU ELISA was used to detect the proliferation of VSMCs, and Western blot was used to detect the protein expressions of RACK1 and phosphor-Akt. Under the static condition, RNA interference was used to suppress the expression of RACK1 in VSMCs, and then the proliferation of VSMCs and expressions of RACK1 and phosphor-Akt were detected. By using co-culture model (ECs/VSMCs) and separated culture model (ECs//VSMCs), the effect of ECs on expressions of RACK1 and phosphor-Akt in VSMCs was further analyzed. Results Comparative proteomic analysis revealed that LowSS increased the expression of RACK1 in rat aorta. In vitro experiments showed that LowSS induced the proliferation, expressions of RACK1 and phospho Akt in VSMCs co-cultured with ECs. Target RNA interference of RACK1 significantly decreased the proliferation of VSMCs, and the phosphorylation of Akt. In comparison with ECs//VSMCs (separated culture) group, the expression of RACK1 and phosphor-Akt were both up-regulated in the VSMCs co-cultured with ECs (ECs/VSMCs group). Conclusions The expression of RACK1 in VSMCs was modulated by shear stress and neighboring ECs, which might induce cellular proliferation via PI3K/Akt pathway. The investigation on VSMC proliferation and the involved biomechanical mechanism will contribute to understanding and help preventing the pathogenesis and progress of atherosclerosis.
