Analytical modeling and optimization of herringbone micro-fluidic chip for capture of circulating tumor cells
10.3871/j.1004-7220.2016.05.401
- VernacularTitle:用于循环肿瘤细胞捕获的鱼骨型微流控芯片的模拟仿真与优化
- Author:
Min-jiao WANG
1
;
Zhi-hua WANG
1
;
Yu-liang DENG
1
;
Qi-hui SHI
1
Author Information
1. Institute for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University
- Publication Type:Journal Article
- Keywords:
Micro-fluidic chip;
Circulating tumor cells (CTCs);
Cell capture;
Fluidic simulation
- From:
Journal of Medical Biomechanics
2016;31(5):E401-E407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To realize the high-efficient capture of circulating tumor cells (CTCs) in the blood of tumor patients by analytic modeling and optimization on the herringbone micro-fluidic chip. Methods By simulating the fluid flow within the herringbone chip with Fluent 15.0 and calculating the capture efficiency with MATLAB to understand how geometric parameters (the herringbone groove width, spacing between herringbone grooves, herringbone groove height and channel height), flow rates and flow directions (forward flow, reverse flow) affected the cell-surface contact for capture of the CTCs, the capture efficiency was predicted and then validated by experiments. Results The herringbone micro-fluidic chip could achieve the optimal capture rate when the herringbone groove width, spacing between herringbone grooves, herringbone groove height and channel height were 75, 125, 70 and 30 μm, respectively, at a flow rate of 1 mL/h with forward direction. Conclusions In this study, cell capture in different micro-fluidic chips was simulated by the method of computational fluid dynamics. The statistic model of capture efficiency is established by MATLAB and optimized to quickly screen a group of physical parameters for high-efficient cell capture. These optimized micro-fluid chip parameters are validated by experiment, which can realize the high-efficient capture of CTCs.
