The influence of mechanical stretch on expression of NLRP3 inflammasome related factors in human periodontal ligament cells
10.3871/j.1004-7220.2016.03.272
- VernacularTitle:牵张应变对人牙周膜细胞中NLRP3炎症体通路相关因子表达的影响
- Author:
Dan ZHAO
1
,
2
;
Ya-qin WU
1
,
2
;
Chun XU
1
,
2
;
Fu-qiang ZHANG
1
,
2
Author Information
1. Department of Prosthodontics，Shanghai Ninth People’s Hospital，Shanghai Jiao Tong University School of Medicine
2. Shanghai Research Institute of Stomatology，Shanghai Key Laboratory of Stomatology
- Publication Type:Journal Article
- Keywords:
Human periodontal ligament cells (HPDLCs);
Mechanical stretch;
Inflammasome;
In vitro culture
- From:
Journal of Medical Biomechanics
2016;31(3):E272-E277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of mechanical stretch on expression of inflammasome related factors in human periodontal ligament cells (HPDLCs). Methods HPDLCs were subjected to mechanical stretch with a 20% elongation magnitude for 6 h or 24 h, respectively. The mRNA and protein expression levels of IL-1β, Caspase-1 and NLRP3 were detected by real-time quantitative PCR and Western blot. The levels of IL-1β in the cell-culture medium of HPLCs in response to mechanical stretch for 1, 2, 4, 6 h were detected by ELISA, respectively. In control group, HPDLCs were cultured in similar conditions but not subjected with stretch. Results The mRNA and protein expression levels of IL-1β, Caspase-1 and NLRP3 were up-regulated with mechanical stretch for 6 h (P<0.05). The protein expression level of NLRP3 was up-regulated with mechanical stretch for 24 h (P<0.05). Compared with control group, the content of IL-1β in the cell-culture medium of HPLCs was increased significantly in response to mechanical stretch for 4 h and 6 h(P<0.05). Conclusions The expression of NLRP3/Caspase-1/IL-1β related factors in HPDLCs can be induced by 20% mechanical stretch for 6 h.
