Study on the correlation of integrin distribution changes with directional migration of hepatoma cells
- VernacularTitle:整合素分布变化与肝癌细胞迁移取向调节的相关性
- Author:
Hong bing WANG
1
;
Qiu hua XU
1
;
Yan ming LIU
1
;
Guang lei YU
2
;
Li YANG
1
;
Ze zhi WU
1
;
BEN Yanzi YANG
1
Author Information
1. Key Laboratory for Biorheology and Technology of Ministry of Education, College of Bioengineering, Chongqing University
2. College of Mathematics and Physics, Chongqing University
- Publication Type:Journal Article
- Keywords:
Cell adhesion;
Mechanical stretch;
Cell culture;
Integrins distribution;
Cytoskeleton
- From:
Journal of Medical Biomechanics
2010;25(4):E288-E295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of integrin redistribution on hepatoma cell alignment and migration and the influence of cytoskeleton reassembly on integrin redistribution by the method of mechanical loading unloading and fibronection(FN) coating. Method By using immuneofluescence staining, cofocal laser scanning microscopy and quantitative morphological analysis, integrin distribution change and crtoskeleton assembly adjustment were observed and the deformation of cell movement was tested and analyzed quantitatively. Results (1) cells with different forms have different integrin expressions and distribution features. The β1 integrin expression for spreading cells was higher than that for round (nonspreading) cells. For spreading cells, the strongest staining was found towards the attachment surface. While for round (nonspreading) cells, the integrin staining on the free surface towards medium was stronger than that towards the attachment surface. (2) After 5 hours of mechanical stretch, the β1 integrin expression for both spreading and round cells increased, and distribution peaks towards the attachment surface broadened. At 1 hour after unloading, the β1 integrin expression decreased and the distribution of integrin staining showed the tendency of dispersion, especially for round cells. (3) After coating the substrates with FN, the β1 integrin expression increased. The integrin staining for either spreading or round cells was more towards the attachment surface to reduce the migration of hepatoma cells. 4) After 5 hours of mechanical stretch, 60% of cells showed their orientation of major axes distributed between 70°～110° towards the stretching direction, and the cytoskeleton aligned vertically to the stretching direction. Cytoskeletons were found significantly depolymerized at 1 hour of unloading. Conclusions The change of integrin distribution is affected by cytoskeleton aligned and the number of ligand. The distribution feature of the whole integrin expression on the surface of individual round cells is related to their stronger invasion and metastasis capability.
