Effects of Yiqi Yangyin Liangxue method on CD4+ CD25+ regulatory T cells in immune thrombocytopenia purpura mice model
10.3760/cma.j.issn.1008-6706.2019.23.001
- VernacularTitle: 益气养阴凉血方对免疫性血小板减少性紫癜模型小鼠CD4＋CD25＋调节性T细胞的影响
- Author:
Lian HU
1
;
Songshan LIU
1
;
Bingjie ZHAO
1
;
Hong CHE
1
;
Yi WANG
1
;
Li XIAO
1
;
Xuemei WANG
2
;
Qian ZHAO
2
;
Peijia LIU
2
;
Yaling HE
2
Author Information
1. Department of Hematopathy, the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610072, China
2. Graduate Student 2016 of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610072, China
- Publication Type:Journal Article
- Keywords:
Purpura, thrombocytopenic;
Immunity;
Drugs, Chinese herbal;
Medicine, Chinese traditional;
Glucocorticoids;
T-Lymphocytes;
Mice;
Comparative effectiveness research
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(23):2817-2821
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Yiqi Yangyin Liangxue method on peripheral blood platelet count and CD4+ CD25+ regulatory T cells(Treg) in mice model of immune thrombocytopenic purpura(ITP).
Methods:A total of 100 mice were randomly divided into blank control group, model group, Chinese medicine group, hormone group, Chinese medicine+ hormone group, with 20 mice in each group.In addition to the blank control group, the other four groups were intraperitoneally injected with guinea pig anti-mouse platelet serum(APS) to establish the ITP model.The peripheral blood platelet counts of mice in each group were determined by animal blood analyzer before modeling, before and after gavage, and CD4+ CD25+ Treg cells in each group were detected by flow cytometry after gavage.
Results:After intragastric administration, compared with the model group, the peripheral blood platelet count of mice in the other groups increased significantly[(413.55±38.84)×109/L, (710.45±124.52)×109/L, (768.10±127.42)×109/L, (908.05±89.66)×109/L, t=-10.18, -11.90, -22.63, all P<0.01], and the proportion of CD4+ CD25+ Treg cells in peripheral blood increased significantly[(1.649±0.286)%, (2.000±0.193)%, (2.286±0.271)%, (2.648±1.883)%, t=-4.54, -7.221, -13.031, all P<0.01]. All indicators of the Chinese medicine+ hormone group had statistically significant differences compared with those of the Chinese medicine group and the hormone group(t=-5.759, -4.107, -10.762, -4.902, all P<0.01).
Conclusion:Yiqi Yangyin Liangxue method may up-regulate the expression of CD4+ CD25+ Treg cells in peripheral blood of ITP mice, thereby reducing excessive platelet damage.
