Levels of miRNA-106a in non-small cell lung cancer tissues and its clinical significance
10.3760/cma.j.issn.1008-6706.2019.20.017
- VernacularTitle: 非小细胞肺癌组织中微核糖核酸-106a表达水平及临床意义
- Author:
Zaohong LI
1
Author Information
1. Department of Pathology, Yiwu Central Hospital, Yiwu, Zhejiang 322000, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, non-small-cell lung;
tissue;
Ribonucleases;
miRNA-106a;
Prognosis;
Real-time fluorescence quantitative polymerase chain reaction;
Diagnosis;
Survival;
Recurrence
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(20):2510-2514
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the level and clinical significance of miRNA-106a in non-small cell lung cancer tissues.
Methods:Paraffin-embedded specimens of non-small cell lung cancer tissues and adjacent tissues from 80 patients with non-small cell lung cancer from January 2012 to December 2013 in Yiwu Central Hospital were selected in this study.The real-time fluorescence quantitative polymerase chain reaction (QRT-PCR) was used to determine the level of miRNA-106a in lung cancer tissues and adjacent tissues.
Results:The level of miRNA-106a in non-small cell lung cancer tissues (2.42±0.23) was higher than that in adjacent tissues (1.00±0.06) (t=53.433, P=0.000). The miRNA-106a levels in lung cancer tissues of stage Ⅲ-Ⅳ, lymph node metastasis and recurrence time<6 months were high than those of the stage Ⅰ-Ⅱ, no lymph node metastasis and recurrence time ≥ 6 months(t=7.641, 11.115, 2.183, P=0.000, 0.000, 0.032). The level of miRNA-106a was not associated with age, gender, pathological type, degree of differentiation and vascular invasion (all P>0.05). The level of miRNA-106a in non-small cell lung cancer tissues was cut by 2.12.The area under the ROC curve for the diagnosis of non-small cell lung cancer was 0.823(95% CI=0.820-0.825, P=0.000), and the sensitivity was 54.37%, the specificity was 89.21%.The cumulative survival rate and progression-free survival rate of patients with low-expression of miRNA-106a in non-small cell lung cancer were higher than those with high expression of miRNA-106a (P=0.027, 0.012).
Conclusion:The miRNA-106a level is elevated in non-small cell lung cancer tissues.The miRNA-106a may be involved in the development of non-small cell lung cancer and is of great value in the diagnosis and prognosis evaluation of non-small cell lung cancer.
