Study on urine metabolomics of left-behind children with vitamin D deficiency under 1 year old in Zunyi area based on nuclear magnetic resonance
10.3760/cma.j.issn.2095-428X.2019.20.011
- VernacularTitle: 基于磁共振技术对遵义地区1岁以下维生素D缺乏症留守儿童尿液的代谢组学研究
- Author:
Li LEI
1
;
Yu CHENG
2
;
Yanan SONG
2
;
Yunfeng XIANG
2
;
Hongjiao JIN
1
;
Huiling SONG
2
;
Enjin GOU
1
;
Qing LI
1
;
Xuqin WANG
1
;
Limei LUO
1
;
Yong LIN
1
;
Bo HUANG
1
Author Information
1. Department of Pediatric Intensive Medicine, the Third Affiliated Hospital of Zunyi Medical University(the First People′s Hospital of Zunyi), Zunyi 563000, Guizhou Province, China
2. Graduate School of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
- Publication Type:Journal Article
- Keywords:
Vitamin D deficiency;
Nuclear magnetic resonance;
Metabolomics;
Differential metabolites
- From:
Chinese Journal of Applied Clinical Pediatrics
2019;34(20):1565-1569
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the characteristic changes in urinary metabolites in left-behind children with vitamin D deficiency under 1 year old in Zunyi area by metabolomic nuclear magnetic resonance (NMR) in order to provide new biomarkers for early diagnosis of vitamin D deficiency.
Methods:From January to August 2018, blood tests and urine collection were carried out on the left-behind children under 1 year old in Fenggang county, Bozhou district and Zheng′an county under Zunyi city by stratified sampling.Forty children diagnosed as a vitamin D deficiency were selected as a vitamin D deficiency group, and 40 children with normal urine test were selected as a healthy control group.For urine sampling, SIMCA-P+ software was applied to analyze the integral value of hydrogen spectrogram by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) was used to distinguish the difference in urine metabolites between two groups of the left-behind children.Orthogonal partial least squares discriminant analysis (OPLS-DA) was used to screen different metabolites.
Results:The serum level of 25-hydroxy vitamin D[25-(OH)D][(32.0±3.6) nmol/L ] in the healthy control group was higher than that in the vitamin D deficiency group[(15.8±2.3) nmol/L], and the difference was statistically significant (P<0.05). PCA and PLS-DA analysis showed significant differences in urine metabolites between the healthy control group and the vitamin D deficiency group (P<0.05). OPLS-DA indicated R2X=0.365, Q2=0.978, which further verified the difference of metabolites.Compared with the healthy control group, the urine of methyl malonic acid, 3-hydroxy butyrate, N-acetyl glycoprotein signal, glutamic acid, dimethyl glycine, 2-ketone glutaric acid, taurine, fumaric acid salt level increased significantly in the vitamin D deficiency group, and the differences were statistically significant (|r|>0.602, all P<0.05, df=39). However, the levels of ethyl malonic acid, creatine, choline, glycerophosphalocholine and equine were significantly decreased, and the differences were statistically significant (|r|>0.602, all P<0.05, df=39).
Conclusions:The left-behind children under 1 year old with vitamin D deficiency in Zunyi region are mainly characterized by disorder in energy metabolism, lipid metabolism, amino acid metabolism and intestinal microbial meta-bolism disorders, and their differential metabolites have potential application value in early diagnosis and pathogenesis of vitamin D deficiency.
