Effect of hyperoxia exposure on expression of microRNA-125b and tumor necrosis factor alpha, interleukin-6 in lung tissues of premature newborn rats
10.3760/cma.j.issn.2095-428X.2019.16.013
- VernacularTitle: 高体积分数氧暴露对早产新生大鼠肺组织微小RNA－125b、肿瘤坏死因子－α和白细胞介素6表达的影响
- Author:
Xiaoyue ZHANG
1
;
Cheng CAI
;
Xiaoyun CHU
;
Huilin ZHOU
Author Information
1. Department of Neonatology, Children′s Hospital Affiliated to Shanghai Jiaotong University, Shanghai Children′s Hospital, Shanghai 200062, China
- Publication Type:Journal Article
- Keywords:
Hyperoxia exposure;
Rat, premature;
Lung injury;
MicroRNA-125b;
Tumor necrosis factor alpha;
Interleukin-6
- From:
Chinese Journal of Applied Clinical Pediatrics
2019;34(16):1244-1248
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression of microRNA-125b (miR-125), tumor necrosis factor alpha (TNF-α) and interleukin-6(IL-6) in premature rats exposed to hyperoxia.
Methods:Eighty 1-day old Sprague Dawley (SD) rats were randomly divided into an air group and a hyperoxia group.The rats in the hyperoxia group were continuously exposed to oxygen chamber for 1-3 L/min, oxygen volume fraction was maintained at (800±50) mL/L, and the rats in air group were placed in the same room with the oxygen volume fraction at 210 mL/L.The feeding conditions were same in 2 groups.Lung tissues of premature rats were extracted at different time (1, 4, 7, 10, 14 days). The pathologic changes in the lung tissues were observed by hematoxylin-eosin (HE) staining.The levels of miR-125b and TNF-α, IL-6 in lung tissues were detected by reverse transcription polymerase chain reaction (qRT-PCT) and enzyme-linked immunosorbent assay (ELISA).
Results:Compared with the air group, miR-125b in the hyperoxia group increased slowly after day 1, reached the highest in day 10 (2.554±0.323), and the relative expression in day 14 decreased slightly(2.329±0.263), and there were significant differences between 2 groups at di-fferent time (all P<0.05); in particular TNF-α level increased in day 7 [(78.55±39.53) ng/L], and reached the peak at day 10 [(80.16±11.24) ng/L], and there was a significant difference(P<0.05); IL-6 levels increased at day 7 [(45.44±31.94) ng/L], and reached the peak at day 10 [(90.38±8.24) ng/L], and there was a significant difference(P<0.05). There was a no significant correlation between miR-125b and TNF-α in the hyperoxia groups (r=0.132, P>0.05), but there was significant correlation between miR-125b and IL-6 in hyperoxia groups(r=0.439, P<0.05).
Conclusions:The levels of miR-125b, TNF-α and IL-6 are involved in the pathological process of bronchopulmonary dysplasia induced by hyperoxia, and IL-6 may be the key factor for miR-125b.
