Protective effect and mechanism of glucose regulatory protein on atrial fibrillation
10.3760/cma.j.issn.1008-6706.2019.14.012
- VernacularTitle: 葡萄糖调节蛋白对心房颤动心肌细胞的保护作用及机制研究
- Author:
Xuefeng TIAN
1
;
Zheng WANG
;
Lin CHEN
;
Sining LIU
;
Yue ZHANG
;
Zhihong ZHANG
;
Mingyang HONG
Author Information
1. Department of Cardiovascular Diseases, Heilongjiang Provincial Hospital, Haerbin, Heilongjiang 150001, China
- Publication Type:Journal Article
- Keywords:
Glucose regulatory protein;
Atrial fibrillation;
Oxidative stress
- From:
Chinese Journal of Primary Medicine and Pharmacy
2019;26(14):1708-1712
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of glucose regulatory protein 78 (GRP78) on cardiomyocytes in atrial fibrillation and its mechanism.
Methods:HL-1 cardiomyocyte cell line was cultured from June 2016 to March 2017 at Harbin Medical University Central Laboratory.The cells were randomly divided into 4 groups: control group, pacing group, pacing+ GRP78cDNA group, pacing+ GRP78siRNA group.The following indicators were detected: (1)oxidative stress: flow cytometry was used to detect the content of reactive oxygen species (ROS) in each group; (2)Ca2+ overload and cell electrophysiology: flow cytometry was used to detect Ca2+ content in each group; (3)cell structure reconstruction: cell apoptosis was detected by flow cytometry.
Results:The ROS production in the pacing group was significantly higher than that in the control group [(28.8±0.5)% vs.(4.5±0.8)%, F=27.91, P<0.01]. The number of ROS in the GRP78siRNA group was significantly higher than that in the pacing group [(71.1±0.3%) vs.(28.8±0.5)%, F=34.12, P<0.01]. The ROS content of the GRP78cDNA group was lower than that of the pacing group [(26.1±2.7)% vs.(28.8±0.5)%, F=26.60, P<0.01]. Pacing significantly increased Ca2+ concentration in HL-1 cells; GRP concentration in HL-1 cells in the GRP78siRNA group was significantly higher than that in the pacing group [(72.53±2.5)AM vs.(39.23±1.9)AM, F=19.50, P<0.05]. The intracellular Ca2+ concentration in the GRP78cDNA group was significantly lower than that in the pacing group [(31.23±0.57)AM vs.(39.23±1.9)AM, F=28.17, P<0.01]. The survival rate of the GRP78cDNA group was significantly higher than that of the pacing group [(87.9±0.4)% vs.(80.1±0.5)%, F=19, P<0.05].
Conclusion:GRP78 protein reduces intracellular calcium overload by reducing ROS production in myocardial cells during atrial fibrillation.It can alleviate the oxidative stress of cardiomyocytes and improve the survival rate of cardiomyocytes, and has protective effect on cardiomyocytes.
