Effect of Tacrolimus on the expression of α-actinin-4 in podocytes induced by Puromycin aminonucleoside
10.3760/cma.j.issn.2095-428X.2019.13.010
- VernacularTitle: 他克莫司对嘌呤霉素损伤的足细胞中α-辅肌动蛋白-4表达的影响
- Author:
Jieying FENG
1
;
Li YU
;
Zhihong HAO
;
Shengyou YU
Author Information
1. Department of Pediatrics, Guangzhou First People′s Hospital, Guangzhou Medical University, Guangzhou 510180, China
- Publication Type:Journal Article
- Keywords:
α-actinin-4;
Tacrolimus;
Podocytes;
Puromycin aminonucleoside
- From:
Chinese Journal of Applied Clinical Pediatrics
2019;34(13):1006-1010
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Tacrolimus(FK506) and Puromycin aminonucleoside(PAN) on apoptosis and expression of α-actinin-4 mRNA and protein in mouse glomerular podocytes in order to explore the protective effect of FK506 on podocytes.
Methods:Mouse glomerular podocytes were cultured in vitro, and the control group, PAN group and FK506 group were established.After 8 h, 24 h and 48 h of treatment, the cell morphology was observed and the apoptosis rate was detected.The cells were collected by real-time PCR and Western blot was used to detect the mRNA and protein expression of α-actinin-4.
Results:The cell body area of the PAN group was significantly smaller than that of the control group, and the cell area of the FK506 group was larger than that of the PAN group.There was no significant difference in the rate of podocyte apoptosis between those groups at 8 h (all P>0.05). At 24 h and 48 h, the apoptotic rate of podocytes in PAN group[(8.21±0.41)%, (16.32±0.17)%] were significantly higher than those in the control group[(4.28±0.35)%, (6.27±0.28)%], and the differences were significant (all P<0.05). The apoptosis rate of podocytes in FK506 group[(6.26±0.24)%, (13.32±0.24)%] were significantly lower than those in PAN group, and the differences were significant (all P<0.05). At 8 h, there was no significant difference in the expression of α-actinin-4 mRNA and protein(all P>0.05). The expression of mRNA (2.42±0.21, 3.78±0.25) and protein(0.77±0.04, 1.22±0.10) in the PAN group was significantly higher than mRNA(1.50±0.22, 2.15±0.15) and protein(0.44±0.03, 0.83±0.07) in the control group at 24 h and 48 h, and the differences were significant (all P<0.01). The expression of mRNA (1.65±0.24, 1.70±0.32) and protein (0.52±0.05, 0.56±0.07) in FK506 group was significantly lower than that of PAN group, and the differences were significant (all P<0.05).
Conclusions:FK506 can effectively inhibit the damage of PAN on podocytes and stabilize the expression of α-actinin-4, which provides a basis for the clinical application of FK506 in the treatment of glomerular diseases.
