Extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma in Delaying High Glucose-induced Vascular Senescence in Mice
10.13422/j.cnki.syfjx.20190439
- VernacularTitle: 人参-三七-川芎提取物延缓高糖诱导的小鼠血管衰老的机制探讨
- Author:
Jing-yi FANG
1
;
Xue WANG
2
;
Yan LEI
1
;
Jing YANG
2
;
Cheng-kui XIU
2
;
Wei TIAN
3
Author Information
1. Research Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China
2. Experimenalt Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China
- Publication Type:Research Article
- Keywords:
high glucose;
vascular aging;
extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma;
AMP-activated protein kinase(AMPK);
mechanistic target of rapamycin(mTOR)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):81-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effect of extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma on vascular senescence induced by high glucose in mice from adenosine 5'-monophosphate (AMP)-activated protein kinase/mechanistic target of rapamycin (AMPK/mTOR) pathway. Method: A total of 130 male C57BL/6 mice were randomly divided into control group and high glucose group. The high glucose group was intraperitoneally injected with streptozocin(STZ) and fed with a high-fat diet continuously for seven months. Mice were divided into 4 groups:model group, low-dose extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma(0.819 g·kg-1) group, high-dose extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma group (1.638 g·kg-1) and metformin group (150 mg·kg-1), and intragastrically administered once a day for nine weeks. The changes in body weight and blood glucose were measured. At the end of the administration, htoxylin eosin(HE) was performed for the detection of aortic morphology, and the expressions of cyclin-dependent kinase inhibitor 2A (p16), cyclin-dependent kinase inhibitor 1A (p21), AMPK, p-AMPK, mTOR, p-mTOR, liver kinase B1 (LKB1), p-LKB1, Ribosomal protein s6 kinase (p70s6k) and p-p70s6k proteins in mouse aorta were detected by Western blot. Result: Compared with blank group, mice in model group had lower body weight and higher blood glucose (P<0.01). After 9 weeks of drug intervention, there was no significant difference in body weight among groups, and the blood glucose level was significantly lower than that in model group (P<0.05, P<0.01). Model group showed a severe intima injury and hyperplasia, middle membrane was obviously proliferated and irregularly arranged. After drug intervention, the intimal damage of each group was not obvious, and the middle membrane was slightly proliferated. The protein expressions of p16, p21, mTOR, p-mTOR, p70s6k and p-p70s6k in model group were significantly higher than those in control group (P<0.05, P<0.01), and the protein expressions of AMPK, p-AMPK, LKB1 and p-LKB1 were significantly decreased (P<0.05, P<0.01). After drug intervention, the protein expressions of p16, p21, mTOR, p-mTOR, p70s6k, p-p70s6k in each group were significantly decreased (P<0.05, P<0.01), while the protein expressions of AMPK, p-AMPK, LKB1, p-LKB1 were significantly increased (P<0.05, P<0.01). Conclusion: High glucose can induce vascular senescence, and extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma can improve vascular aging induced by high glucose through AMPK/mTOR pathway.
