Mechanism of Modified Maimendong Tang Combined with Cisplatin in Enhancing Chemosensitivity on Human Lung Adenocarcinoma A549 Cells
10.13422/j.cnki.syfjx.20190217
- VernacularTitle: 麦门冬汤加减联合顺铂对A549细胞化疗增敏的作用机制
- Author:
Fang FANG
1
;
Ling-li XIE
1
;
Xue-jian NING
2
;
Xian-ji FAN
2
;
Wan-yin WU
1
Author Information
1. Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2. Guangxi Zhuang Autonomous Region Liuzhou Hospital of Traditional Chinese Medicine(TCM), Liuzhou 545000, China
- Publication Type:Research Article
- Keywords:
modified Maimendong Tang;
cisplatin;
A549 cells;
apoptosis;
cell invasion and metastasis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):49-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe effect of modified Maimendong Tang combined with cisplatin on the cell proliferation, apoptosis, invasion and metastasis and the protein expressions of Caspase-3, epidermal growth factor receptor(EGFR)of human lung adenocarcinoma A549 cells in vitro, so as to investigate their relevant mechanisms in inhibiting cells proliferation, invasion and metastasis and inducing apoptosis of A549 cells. Method: The lung cancer cells A549 were respectively treated with modified Maimendong Tang(15 g·L-1), cisplatin(9 mg·L-1), and combined drugs. Afterwards, they were divided into control group, modified Maimendong Tang group, cisplatin group and modified Maimendong Tang combined with cisplatin group. Thiazolyl blue tetrazolium bromide (MTT) assay was used to evaluate the proliferation of A549 cells treated with different concentrations of modified Maimendong Tang(0, 5, 10, 15, 20, 25 g·L-1) and cisplatin(0, 3, 6, 9, 12, 15 mg·L-1) for 24, 48, 72 h. The proliferation of A549 cells in each group was detected by MTT assay; flow cytometry was used to detect the degree of apoptosis and cycle in the above four groups of cells; scratch test and transwell migration test were performed to observe the abilities of invasion and metastasis of each group; Western blot was used to detect Caspase-3 and EGFR protein expression. Result: The concentration of modified Maimendong Tang and cisplatin and the time of intervention were negatively correlated with the proliferative capacity of A549 cells (P<0.05); compared with control group, modified Maimendong Tang could reduce the expressions of Caspase-3 and EGFR obviously (P<0.05); compared with cisplatin group and modified Maimendong Tang group, the modified Maimendong Tang combined with cisplatin group significantly inhibited the proliferation of lung cancer cell and induced its apoptosis, the number of cells in G0/G1 phase increased apparently, and the cells in S phase decreased significantly (P<0.05); compared with other groups, A549 cells treated with the combined drug showed the poorest ability of scar healing, the number of them passing through the transwell chamber significantly reduced, and the protein expressions of Caspase-3 and EGFR down-regulated (P<0.05). Conclusion: Modified Maimendong Tang combined with cisplatin can inhibit the proliferation, invasion and metastasis and induce the apoptosis of lung cancer cells. Modified Maimendong Tang can synergistically enhance the action of cisplatin. The mechanism may be related to the down-regulation of Caspase-3 and EGFR protein expressions.
