Effect of Zingiberis Rhizoma Recens on Hemorrhoids Model in Rats and Mice
10.13422/j.cnki.syfjx.20190440
- VernacularTitle: 生姜外用对大、小鼠痔疮模型的影响
- Author:
Xin LOU
1
;
Ming BAI
1
;
Shuo TIAN
1
;
Xiang-ping WEI
1
;
Ming-san MIAO
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
external application;
hemorrhoids model;
acetic acid solution;
Zingiberis Rhizoma Recens;
Yongquan acupoint
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):23-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of Zingiberis Rhizoma Recens(ZRR) on hemorrhoids in mice and rats. Method: Sixty SPF-grade SD rats were divided into blank group, model group, Ma Yinglong Shexiang hemorrhoid ointment group (7.5 g·kg-1), and large and small-dose ZRR paste groups (10, 5 g·kg-1). ZRR paste was applied in Yongquan acupoint to observe the effect of 0.05 mL injection with 75% acetic acid solution on hemorrhoids induced by subcutaneous anus in rats, the levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), nitric oxide (NO) in the serum were detected by enzyme linked immunosorbent assay(ELISA), and the rectal histopathology was detected by hematoxylin-eosin(HE). Sixty SPF-grade KM mice were divided into blank group, model group, MA Ying-long Shexiang hemorrhoid ointment group (7.5 g·kg-1), and large and small-dose ginger essential oil groups (0.06, 0.03 mL, three times a day). ZRR paste was applied in crissum to observe the effect of injection of 20% acetic acid solution 0.05 mL (maintaining for 1 min) on hemorrhoids in mice induced by anus. The degree of local swelling ulcer around the anus and score was observed, levels of IL-1β, TNF-α in the serum were determined by ELISA, and the rectal histopathology was detected by HE staining. Result: In the experimental study on treating hemorrhoids with ZRR paste applied on Yongquan point of rats, compared with normal group, serum levels of IL-1β, IL-6, TNF-α, NO in model group were significantly higher (P<0.01). The pathological injury of rectum was serious. Compared with model group, serum levels of IL-1β, IL-6, TNF-α, NO were decreased in each administration group (P<0.05, P<0.01), and the degree of rectal tissue injury was relieved (P<0.01). In the experiment of external application of ZRR essential oil around the anus for treating hemorrhoids, compared with normal group, perianal swelling and severe ulcer injury were observed in the model group (P<0.01). The serum levels of IL-1β, TNF-α in model group were significantly higher (P<0.01), and the pathological injury of rectum was serious. Compared with model group, each administration group showed the alleviation in perianal pathological conditions (P<0.01), the reduction in serum IL-1β, TNF-α levels (P<0.01), and the relief in the extent of rectal tissue injury (P<0.01). Conclusion: External application of ZRR can effectively inhibit perianal swelling and ulcer degree, with a good therapeutic effect on hemorrhoids model in rats and mice.
