Effect of Sanshencao Essential Oil on Rat Model of Thromboangiitis Obliterans
10.13422/j.cnki.syfjx.20190438
- VernacularTitle: 三参草精油对大鼠血栓闭塞性脉管炎模型的影响
- Author:
Jing LIU
1
;
Shuo TIAN
1
;
Ming-san MIAO
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
Sanshencao essential oil;
thromboangiitis obliterans;
external treatment of Chinese medicine;
treatment mechanism;
aromatherapy
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(4):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of Sanshencao essential oil on the model of thromboangiitis obliterans in rats. Method: Male Wistar rats were randomly divided into five groups according to body weight:sham operation group, model group, Tongsaimai group (1.05 g·kg-1), large and small-dose Sanshencao essential oil groups (0.125 mL·cm-2, twice a day, 0.125 mL·cm-2, once a day), with 10 in each group. The model of occlusive vasculitis was established by injecting sodium laurate solution (10 g·L-1) into the left lower limb artery of rats. Sham operation group was injected with the same amount of normal saline at the same position. Large and small doses of Sanshencao essential oil were applied directly to the affected area, and the remaining groups were given corresponding drugs or pure water for 10 days. One hour after the last administration, the pathological changes were graded; the levels of thromboxane B2(TXB2), interleukin-1 (IL-1), 6-oxo-prostaglandinF1α (6-K-PGF1α), endothelin (ET) were measured by radioimmunoassay. Hematoxylin-eosin(HE) staining was used to observe the pathological changes of the femoral arteries in the left lower limbs. Result: Compared with sham operation group, the degree, extent, inflammation and thrombosis of the affected limbs in model group increased significantly (P<0.05, P<0.01). Compared with model group, large and small-dose Sanshencao essential oil could significantly alleviate the extent, inflammation and thrombosis of the affected limbs (P<0.05, P<0.01), decrease the levels of TXB2, ET and IL-1 in serum (P<0.05, P<0.01), and increase the level of 6-K-PGF1α (P<0.05, P<0.01). Conclusion: Sanshencao essential oil has a good therapeutic effect on thrombosis angiitis obliterans model in rats, which related to its anti-oxidation and alleviating inflammation of vascular wall.
