HPLC Fingerprint Analysis and Chemistry Pattern Recognition of Wild and Cultivated Notholirion bulbuliferum from Different Regions
10.13422/j.cnki.syfjx.20190215
- VernacularTitle: 野生与栽培太白米HPLC指纹图谱及化学模式识别
- Author:
Bing-yue YANG
1
;
Ben-xiang HU
1
;
Lin ZHANG
1
;
Gang ZHANG
1
;
Yong-gang YAN
1
;
Yi-min LI
1
;
Jing GAO
1
;
Wei WANG
1
;
Mei WANG
1
;
Liang PENG
1
Author Information
1. College of Pharmacy, Shaanxi University of Chinese Medicine, Xi'an 712046, China
- Publication Type:Research Article
- Keywords:
Notholirion bulbuliferum;
HPLC fingerprint;
wild;
cultivated
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):187-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the HPLC fingerprint of wild and cultivated Notholirion bulbuliferum,and recognize them according to the chemical pattern, in the expectation of providing the basis for the quality control and domestication cultivation of N. bulbuliferum of origins. Method: Twenty samples of wild and cultivated N. bulbuliferum collected from different origins were detected by HPLC, and a common mode of fingerprint was established. The Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012A edition) was used to evaluate the similarity of the samples. The differences among the samples were identified by chemical pattern recognition methods, including principal component analysis (PCA),cluster analysis (HCA) and partial least squares discriminate analysis (PLS-DA). Result: The HPLC fingerprint of N. bulbuliferum was obtained,and 26 common peaks were found in the chromatograph. Similarities of all samples were over 0.9,PCA,and HCA and PLS-DA results demonstrated obvious distinctions between wild and cultivated N. bulbuliferum. Eight constituents,such as pcoumaric acid were identified as biomarkers,representing major differences between the two varieties. Conclusion: The HPLC chromatogram of N. bulbuliferum developed in this paper has strong characteristics and repeatability. After being combined with the pattern recognition mode, it can be used as an effective method for evaluating the quality of N. bulbuliferum and distinguishing wild and cultivated N. bulbuliferum,and provide a reference for the quality control and domestication introduction of N. bulbuliferum.
