Determination of Total Polysaccharides in Decoction Pieces of Polyporus with Different Sources and Different Grades
10.13422/j.cnki.syfjx.20182112
- VernacularTitle: 不同来源、不同等级猪苓饮片中总多糖的含量测定
- Author:
Ning GUO
1
;
Zhi-yong LE
2
;
Wan-wan WANG
1
;
Wei-juan JIA
1
;
Zong-li BAI
2
;
Jian-hua SUN
2
;
Hong WANG
1
Author Information
1. School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
2. Kangmei Pharmaceutical Co. Ltd., Puning 515300, China
- Publication Type:Research Article
- Keywords:
Polyporus;
total polysaccharides;
dextran;
anthrone-sulfuric acid colorimetry;
relative molecular weight;
high performance gel chromatography;
commercial grade
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):156-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for determining the content of total polysaccharides in decoction pieces of Polyporus,analyze the content of total polysaccharides in samples with different sources and grades. Method: The relative molecular weight and the polydispersity index of polysaccharides in decoction pieces of Polyporus were measured by a high performance gel chromatography coupled with a multi-angle laser light scattering and refractive index system.Dextran with similar molecular weight as polysaccharides was selected as the reference substance.Orthogonal experiment and single factor tests were used to optimize the pretreatment conditions for the determination of total polysaccharides in Polyporus.Polysaccharides in Polyporus with different areas and grades were determined by anthrone-sulfuric acid colorimetric method at 630 nm. Result: The linearity,stability,precision,repeatability and recovery rate of the established method all reached the standards,respectively.The content of total polysaccharides in samples from different areas ranged from 0.87% to 1.39%.The content of total polysaccharides in samples with different grades was 1.40% for first-grade pieces,1.21% for second-grade pieces, and 1.03% for third-grade pieces. Conclusion: The established method is simple,accurate and reproducible,and it can be used for the determination of polysaccharides in decoction pieces of Polyporus.The content of polysaccharides in samples from different origins varies greatly.The content of polysaccharides in samples with different grades shows a certain regularity.The content of polysaccharides is the highest in the first-grade pieces,followed by the content in the second-grade,and the lowest in the third-grade.The results can provide a reference for formulating limits for the content of total polysaccharides and the grade standard of decoction pieces of Polyporus.
