Effect of Dodder Total Flavone on Polycystic Ovary Syndrome Rat Models
10.13422/j.cnki.syfjx.20190506
- VernacularTitle: 菟丝子总黄酮对多囊卵巢综合征大鼠模型的影响
- Author:
Ming-san MIAO
1
;
Meng-fan PENG
1
;
Xiao-li YAN
1
Author Information
1. Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
- Publication Type:Research Article
- Keywords:
polycystic ovary syndrome;
apoptosis proteins;
dodder total flavone;
biochemical indicators
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):143-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of dodder total flavone on polycystic ovary syndrome (PCOS) rat models induced by dehydroepiandrosterone (DHEA) combined with human chorionic gonadotropin (HCG). Method: Except the blank group, the remaining rats were injected with DHEA 0.06 mg·g-1 in the morning on the nucha and 1.5 U HCG in the afternoon for 21 consecutive days. On the 16th day after the modeling, the vaginal smear was performed to monitor the estrus cycle. Sixty rats with successful modeling were selected and divided into model group, dacin-35 group, and high, middle and low-dose dodder total flavonoids groups, with 10 rats in each group. On the day of grouping, drugs were given respectively to the drug treatment groups, and the blank group and the model group were given equal volume solvent. The drugs were given continuously for 21 days. Blood was collected from abdominal aorta 2 h later after the final administration, serum levels of testosterone (T), estrogen (E2), luteinizing hormone (LH) and follicle stimulating hormone(FSH) were measured; rats were put to death, and the ovaries at the same part of each rat were fixed in 10% formalin solution. htoxylin eosin (HE) staining was performed, and the morphological changes in the ovaries were observed by light microscopy; the same part of the ovary was taken, and androgen receptor (AR), B-cell lymphoma-2(Bcl-2) and Bcl-2-associated X protein(Bax) were measured in the ovary by immunohistochemistry to observe the expressions of relevant proteins in the ovary; the hypothalamus and pituitary were taken, and the expressions of gonadotropin-releasing hormone (GnRH) in hypothalamus and gonadorelin releasing hormone receptor (GnRHR) in pituitary were detected by immunohistochemistry. Result: PCOS rat model was successfully replicated. Serum levels of T, E2 and LH were significantly reduced, and FSH level of PCOS was significantly increased in each drug treatment group (P<0.01). The pituitary GnRHR level was increased to different degrees (P<0.05,P<0.01); AR and Bax levels of the ovary were decreased to different degrees (P<0.05,P<0.01); hypothalamus GnRH level was significantly decreased, and pathological changes in ovarian tissue of PCOS rats were obvious (P<0.01) in each drug treatment group. Conclusion: The PCOS rat model was successfully replicated. Total flavonoids of dodder may play a protective role in PCOS model rats by regulating the secretion of androgen, inhibiting the expression of ovarian apoptotic protein and impacting the hypothalamic-pituitary-ovary axis pathway.
