Effect of Diosgenin on Expressions of PPARγ, C/EBPα and Adipokine Secretion in Aplastic Anemia Mice
10.13422/j.cnki.syfjx.20190503
- VernacularTitle: 穿龙薯蓣皂苷对再生障碍性贫血小鼠PPARγ,C/EBPα及脂肪分泌因子表达的影响
- Author:
Shan ZHANG
1
;
Li-xiang YIN
2
;
Ai-di WANG
1
;
Xin-long SONG
2
;
Le ZHANG
1
;
Bao-shan LIU
1
Author Information
1. General Hospital of Tianjin Medical University, Tianjin 300052, China
2. Graduate School of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
- Publication Type:Research Article
- Keywords:
aplastic anemia;
diosgenin;
peroxisome proliferator activated receptor γ(PPARγ);
CCAAT-enhancer binding protein α(C/EBPα);
Leptin;
Adiponectin
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):126-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of diosgenin on aplastic anemia (AA) mice and peroxisome proliferator activated receptor γ (PPARγ), CCAAT-enhancer binding protein α (C/EBPα), Adiponectin, Leptin, in order to discuss the potential mechanism of bone marrow mesenchymal stem cells in the process of adipemia. Method: BALB/c mice were randomly divided into control group and model group. The model group was established by 60Coy irradiation combined with tail vein infusion with lymphatic suspension cells of DBA/2 mice. After successful evaluation of the model, the mice were randomly divided into 6 groups:model group, low, medium and high-dose diosgenin groups (37.44,74.88,149.76 mg·kg-1·d-1), cyclosporine group (23.5 mg·kg-1·d-1), and tripterygium glycoside group (9.36 mg·kg-1·d-1), and given corresponding drugs by gavage for 14 days. After the intervention, the peripheral blood of mice in each group was detected, and bone marrow smears were collected to evaluate the proliferation of bone marrow. Bone marrow mesenchymal stem cells (BMMSCs) were isolated and cultured by adherent method and induced by adipogenesis. The mRNA and protein expressions of PPARγ, C/EBPα, Adiponectin, Leptin in BMMSCs were detected by quantitative real-time fluorescent quantitative polymerase chain reaction(Real-time PCR) and Western blot. Result: The white blood cell (WBC), hemoglobin (HGB) and blood platelet (PLT) in peripheral blood of model group were significantly lower than those of normal group (P<0.05). The hemogram of each treatment group was higher than that of model group to varying degrees. The middle-dose Dioscorea nipponica group had the most obvious hemogram, with statistically significant differences compared with the model group (P<0.05). Compared with the control group, the mRNA and protein expressions of PPARγ, C/EBPα, Adiponectin and Leptin in BMMSCs of the model group increased significantly (P<0.05). Compared with the model group, the above indexes in the treatment groups were all decreased, the mRNA and protein expressions of PPARγ, C/EBPα, Adiponectin and Leptin in the middle-dose group diosgenin decreased obviously, which was better than those of Tripterygium glycoside group (P<0.05). Conclusion: Diosgenin can promote the recovery of peripheral blood in aplastic anemia mice and improve the hematopoiesis of bone marrow. Diosgenin can reduce the expressions of PPARγ and C/EBPα, the formation of adipocytes and the secretion of Adiponectin and Leptin in adipocytes, and effectively inhibit the process of adipose tissue derived from bone BMMSCs.
