Neuroprotective Effect and Mechanism of Xiaoyaosan on Lipopolysaccharide-induced Nerve Injury in Rat
10.13422/j.cnki.syfjx.20190536
- VernacularTitle: 逍遥散对LPS所致大鼠神经损伤的保护作用机制
- Author:
Bo-yu SHI
1
,
2
;
Rong LIU
1
,
2
;
Zhi-li RAO
1
,
2
;
Xiao-bo LIU
1
,
2
;
Jie LUO
1
,
2
;
Ya-fei JI
1
,
2
;
Qi LIU
1
,
2
;
Nan ZENG
1
,
2
Author Information
1. Chengdu University of Traditional Chinese Medicine, the Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine
2. Key Laboratory of Systematic Research, Development and Utilization of Chinese Medicine Resources in Sichuan Province-Key Laboratory Breeding Base of Co-founded by Sichuan Province and MOST, Chengdu 611137, China
- Publication Type:Research Article
- Keywords:
Xiaoyaosan;
lipopolysaccharide (LPS);
nerve injury;
neuro protection;
mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(5):50-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect and mechanism of Xiaoyaosan on lipopolysaccharide(LPS)-induced nerve injury. Method: The 56 rats were randomly divided into control group, sham group, model group, amitriptyline group (10 mg·kg-1), fluoxetine group (10 mg·kg-1), Xiaoyaosan group high and low-dose (30,15 g·kg-1).The nerve injury model rat were established by LPS injection into lateral ventride, rats were administrated for 14 days by gavage. The levels of brain-derived neurotrophic factor (BDNF) and β-nerve growth factor (β-NGF) in serum were detected by enzyme linked immunosorbent assay(ELISA), and the expressions of BDNF, nerve growth factor (NGF), tropomyosin receptor kinase B (TrkB), tropomyosin receptor kinase A (TrkA), cAMP response element-binding protein (CREB) mRNA in hippocampus and cortex were detected by Real-time PCR.Protoin expression of BDNF, TrkB, CREB, p-CREB, postsynaptic density protein 95 (PSD95), synaptophysin (SYP) in hippocampus and cortex were detected by Western blot. Result: Compared with control group, LPS decreased the level of BDNF and β-NGF in serum(P<0.01),reduced the expression of BDNF, NGF, TrkB,TrkA, CREB mRNA and the expression of BDNF, TrkB, CREB, Phospho-CREB (p-CREB), PSD95, SYP in hippocampus and cortex(P<0.05,P<0.01). Compared with model group, levels of BDNF and β-NGF in serum in Xiaoyaosan high and low-dose group were increased significantly (P<0.05,P<0.01),and expressions of BDNF, NGF, TrkB,TrkA, CREB, PSD95, SYP in hippocampus and cortex were increased significantly (P<0.05, P<0.01). Conclusion: Xiaoyaosan has a certain antagonistic effect on LPS inducednerve injury, which suggests that the effect is related to activate BDNF/NGF-TrkB/TrkA-CREB pathway and upregulated the expression of synaptic protein.
