The Development and Experimental Study of Endothelial Cell Culture Device in vitro
10.16156/j.1004-7220.2019.05.14
- VernacularTitle:体外内皮细胞培养装置的研制与实验研究
- Author:
Yujia LIU
1
;
Hao DING
2
;
Ying ZHANG
1
;
Chuxun SHI
1
;
Zhichao NIE
1
;
Haoyu LIU
1
;
Zehua CHEN
2
Author Information
1. School of Medical Instrument and Food Engineering，University of Shanghai for Science and Technology
2. Shanghai University of Medicine & Health Sciences
- Publication Type:Journal Article
- Keywords:
in vitro culture device;
endothelial cell;
hemodynamics;
stress
- From:
Journal of Medical Biomechanics
2019;34(5):E541-E547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an innovative device for endothelial cell culture in vitro, namely, to develop a vascular endothelial cell culture device based on hemodynamic environment, so as to introduce the development and experimental study of endothelial cell culture device in vitro. Methods A device of dynamic culture system for endothelial cells in vitro on the basis of the existing research was designed with the theory and method of hemodynamics. The shear stress, positive stress and tensile stress existed at the same time in the flow environment. The development and experimental research of the device were described in detail from 5 aspects, such as the development background, structure and composition, design principle, theoretical basis and experimental research. Results The device could accurately simulate the hemodynamic environment of endothelial cells at normal level, with precise control of shear stress in 0-12 Pa range, positive stress in 0-15.96 kPa range, and tensile stress in 0-0.5 MPa range. Conclusions The device can provide a hemodynamic environment which is closer to the physiological conditions of human body, as well as a more ideal experimental environment and means for further exploring the mechanism of vascular intimal injury.
