Tissue Culture and Exercising Seedlings in Scrophularia ningpoensis
10.13422/j.cnki.syfjx.20190912
- VernacularTitle: 玄参组织培养及炼苗技术
- Author:
Ying-jiao LIU
1
;
Shi-feng CHU
2
;
Ting ZENG
1
;
Piao LUO
1
;
Yao-mei HU
1
;
Nai-hong CHEN
1
Author Information
1. College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
2. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
- Publication Type:Research Article
- Keywords:
Scrophularia ningpoensis;
stem section with axillary;
exercising seedlings;
clonal propagation;
plantlet regeneration
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):168-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Stems,petioles,stem sections with axillary and leaves of Scrophularia ningpoensis were taken as the material in vitro to screen out the suitable plantlet regeneration system and optimal exercising seedling conditions. Method:Different explants,hormones and concentrations on the induction and proliferation of cluster bud were studied by L16(45) orthogonal test. One factor analysis of variance (ANOVA) was made on the induction of adventitious buds rooted with different concentrations of hormones. At the same time,different substrates,watering cycles and transition modes were selected to optimize key technologies of exercising seedlings of S. ningpoensis. Result:Stem sections with axillary was the best explant,which was followed by stems,leaves and petioles. The suitable media for the induction of adventitious buds was MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1,with the induction rate of 100.0% and the proliferation multiple of 9.84.The suitable media for root induction was 1/2 MS+IBA 0.2 mg·L-1,with the rooting rate of 100.0% and the number of roots of 39.45.For matrix,they were transplanted with nutrient soil,vermiculite and perlite (5:2:1) as the media,to keep proper matching of fertility,permeability and water retention. The container seedlings can grow well,and the survival rate was more than 95% when they were watered every 2 days,the acclimatization of plantlets took 20 days indoor and 10 days in shaded greenhouses. Conclusion:The clonal propagation system of S. ningpoensis was established to provide an effective way for the efficient,rapid and steady plantlet regeneration,the breeding of high-quality seedlings and the suitable exercising seedling conditions of S. ningpoensis.
