Analysis of HPLC Fingerprint and Multi-component Chemical Pattern Recognition of Plantaginis Semen from Different Producing Areas in Jiangxi Province
10.13422/j.cnki.syfjx.20191355
- VernacularTitle: 江西不同产区车前子药材的HPLC指纹图谱及其多成分化学模式识别分析
- Author:
Chao LI
1
;
Huan YU
1
;
Rou WEN
1
;
Li-ping YAN
1
;
Shou-wen ZHANG
1
;
Qian-feng GONG
1
;
Xiao-ping YUAN
2
Author Information
1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine(TCM), Nanchang 330004, China
2. Jiangxi Tianqitang TCM Decoction Pieces Co. Ltd., Zhangshu 336000, China
- Publication Type:Research Article
- Keywords:
Plantaginis Semen;
fingerprint;
chemical pattern recognition;
active ingredients;
plantamajoside;
galuteolin;
isoacteoside
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):161-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a HPLC fingerprint detection method of Plantaginis Semen, and analyze the samples from different producing areas in Jiangxi province by combining with chemical pattern recognition method, and the contents of five ingredients in Plantaginis Semen were determined. Method:A total of 34 batches of Plantaginis Semen medicinal materials were detected by HPLC. The similarity evaluation was carried out by the 2012 edition of similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine. The chromatographic peak information was used as the data source, and three chemical pattern recognition methods were used to comprehensively analyze the quality of this medicinal herb. Quantitative analysis was performed on the 5 active components, including geniposidic acid, plantamajoside, acteoside, galuteolin and isoacteoside. Result:The similarities between Plantaginis Semen samples from different producing areas in Jiangxi province were >0.86. Orthogonal partial least squares-discriminant analysis (OPLS-DA) could distinguish samples from different producing areas, and be used to determine the chemical components, which had strong correlation with the quality of Plantaginis Semen. The contents of 5 active components in samples from different producing areas were different to some degree, especially in the content of plantamajoside. Conclusion:The established HPLC fingerprint of Plantaginis Semen has strong characteristics, combined with chemical pattern recognition method, it can effectively evaluate the quality of Plantaginis Semen and distinguish its producing areas.
