Protective Effect of Alkaloid A from Acanthi Ilicifolii Herba seu Radix on Drug-induced Liver Injury in Mice
10.13422/j.cnki.syfjx.20191503
- VernacularTitle: 老鼠簕生物碱A对小鼠药物性肝损伤的保护作用
- Author:
Xiu-gui WEI
1
;
Hong-yuan WANG
1
;
Xiu-kun HUANG
1
;
Xue-mei SUN
1
;
Ying-qin LIANG
1
;
Wan-peng XU
1
;
Xing LIN
1
;
Jun LIN
1
Author Information
1. Department of Pharmacology, Guangxi Medical University, Nanning 530021, China
- Publication Type:Research Article
- Keywords:
alkaloid A from Acanthi Ilicifolii Herba seu Radix;
acute liver injury;
acetaminophen
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):103-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of alkaloid A from Acanthi Ilicifolii Herba seu Radix(AAIA) on liver injury model caused by acetaminophen. Method:Mice were randomly divided into normal group, model group, positive drug group (bifendate, 150 mg·kg-1) and high, medium and low-dose AAIA groups (200, 100, 50 mg·kg-1), with 10 in each group. They were given drugs by gavage for 10 days, and fasted for 8 hours after the last administration. Except the normal group, the other groups were intraperitoneally injected with 275 mg·kg-1 acetaminophen to induce acute liver injury model in mice. Six hours later, blood was taken from the eyeball. The body, liver, spleen, kidney and thymus were weighed, and then the corresponding organ indexes were calculated. The kits were used to detect the contents of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum. The contents of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in liver homogenate were determined by ultraviolet spectrophotometry, and the expressions of extracellular regulated protein kinases (ERK1/2) and phosphorylated extracellular regulatory protein kinase (p-ERK1/2) were determined by Western blot. Result:Compared with the normal group, the liver index, serum AST and ALT levels, the production of NO and iNOS in liver homogenate, the expression of p-ERK1/2 protein in liver of the model group increased significantly (P<0.01). Compared with the model group, all of drug groups could reduce the liver index of mice with acute liver injury induced by acetaminophen, the levels of serum AST and ALT, the production of NO and iNOS in liver and homogenate and the expression of p-ERK1/2 protein in liver (P<0.01). however, it had no significant effect on spleen, kidney and thymus of all acetaminophen injection groups. Conclusion:AAIA may protect mice from drug-induced liver injury by reducing AST and ALT levels, down-regulating the expressions of NO and iNOS, and reducing the expression of protein p-ERK1/2.
