Effect and Mechanism of Dendrobii Officinalis Caulis in Intervening and Preventing Myocardial Fibrosis in Rats with Heart Failure and Heart Qi Deficiency Syndrome After Ischemia-reperfusion
10.13422/j.cnki.syfjx.20191502
- VernacularTitle: 铁皮石斛对缺血再灌注后心衰心气虚型大鼠心肌纤维化的抑制作用
- Author:
Qing-min LI
1
;
Wu-lin QU
2
;
Bo-jun CHEN
1
Author Information
1. Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2. Chenzhou First People's Hospital, Chenzhou 423000, China
- Publication Type:Research Article
- Keywords:
Dendrobii Officinalis Caulis;
ischemia-reperfusion;
heart failure and heart Qi deficiency syndrome;
myocardial fibrosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):83-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expressions of cardiac cycle, myocardial pathology, galectin-3 (Gal-3),transforming growth factor-β (TGF-β),Smad homologue 3 recombinant protein (Smad3) in rats with heart failure and heart failure after ischemia-reperfusion, and the intervention effect of Dendrobii Officinalis Caulis (DOC) myocardial fibrosis in model rats. Method:A rat model of heart failure and Qi deficiency was established through ligation of the left anterior descending coronary artery. The rats were divided into blank group, model group, valsartan group (9.43 mg·kg-1) and DOC group (10 mg·kg-1), with 10 in each group. The blank group and the model group were given an equal volume of physiological saline. The changes in left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic dimension(LVESD), left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS) of the cardiac cycle of rats in each group were recorded by high-resolution ultrasound system. The carboxyterrninal propeptide of type I procollagen (PICP), and carboxyterrninal propeptide of type Ⅲ procollagen (PⅢNP)were detected by enzyme-linked immunosorbent assay (ELISA) kit. The morphological changes of myocardial cells were observed by hematoxylin-eosin (HE) staining. The changes of myocardial fiber tissue and collagen were observed by Masson staining. Western blot was used to detect the protein expressions of Gal-3, TGF-β, Smad3. Result:Compared with the blank group, the levels of LVEDD, LVEF, and LVFS were lower in the model group (P<0.05), the levels of LVESD, PICP and PⅢNP were increased (P<0.05), while the expression levels of Gal-3, TGF-β, and Smad3 were decreased (P<0.05). Compared with the model group, LVEDD, LVEF, LVFS of the control group and the experimental group were better than the model group (P<0.05), and LVESD, PICP, PⅢNP levels of the experimental group were lower than the control group (P<0.05), LVESD, PPIC, PⅢNP were lower than those of the model group (P<0.05), expression levels of Gal-3, TGF-β and Smad3 were lower than those in the model group (P<0.05), and the experimental group was lower than the control group (P<0.05). According to HE staining, the morphological changes in myocardial cells in the experimental group were more significant than that in the control group. Masson staining showed that the morphological changes of myocardial fibrous tissue and collagen tissue in the experimental group were more significant than that in the control group. Conclusion:DOC can effectively inhibit myocardial fibrosis in rats with heart failure and heart Qi deficiency syndrome after ischemia-reperfusion. The mechanism may be correlated with the reduction of the expressions of Gal-3, TGF-β and Smad3.
