Identification of Salviae Yunnanensis Radix et Rhizoma and Its Allied Species Based on Chemical Composition by FTIR with Chemometric Analysis and HPLC Fingerprint
10.13422/j.cnki.syfjx.20191519
- VernacularTitle: 基于红外光谱结合化学计量学及HPLC色谱的紫丹参及其近缘种成分差异
- Author:
Jian-yong HU
1
;
Ming-jin MIAO
1
;
Kun WEN
1
;
Li XU
1
;
Bao-zhong DUAN
1
Author Information
1. College of Pharmaceutical and Chemistry, Dali University, Dali 671000, China
- Publication Type:Research Article
- Keywords:
Salviae Yunnanensis Radix et Rhizoma;
closely related species;
fourier transform infrared spectroscopy;
high performance liquid;
chemometrics;
component difference
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(15):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differences of chemical constituents of Salviae Yunnanensis Radix et Rhizoma and its closely related species,and to provide reference for the clinical application of Salviae Yunnanensis Radix et Rhizoma. Method:Fourier transform infrared (FTIR) spectroscopy combined with principal component analysis (PCA),cluster analysis (HCA) and partial least squares discriminant analysis (PLS-DA) were used to study the differences of components between Salviae Yunnanensis Radix et Rhizoma and its closely related species,and high performance liquid chromatography (HPLC) was used to compare the differences of water-soluble components between them. Result:There were some differences between Salviae Yunnanensis Radix et Rhizoma and its closely related species acrcording to FTIR spectroscopy and HPLC fingerprint,especially the water-soluble polar components were more abundant in Salviae Yunnanensis Radix et Rhizoma than other species. The chemical components of Salvia trijuga,S. przewalskii and S. bulleyana were more similar in terms of their genetic relationship. The result showed that the Salviae Yunnanensis Radix et Rhizoma and its closely related species can be clearly distinguished by FTIR combined with chemometrics method. Conclusion:Compared with its closely related species,Salviae Yunnanensis Radix et Rhizoma has a unique chemical composition,which has great therapeutic potential for blood stasis. The FTIR combined with chemometrics model can be used for rapid identification of Salviae Yunnanensis Radix et Rhizoma and its closely related species.
