Isolation and Identification of Chemical Constituents from Cinnamomi Ramulus
10.13422/j.cnki.syfjx.20190712
- VernacularTitle: 桂枝化学成分的分离鉴定
- Author:
Ling-li ZHU
1
;
Zhi-fu AI
2
;
Li XU
3
;
Yong-liang JIN
1
;
Chun-ling LIU
1
;
Feng LIU
4
;
Hua LIU
1
Author Information
1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine(TCM), Nanchang 330004, China
2. Graduate School, Jiangxi University of TCM, Nanchang 330004, China
3. People's Hospital of Zhangshu, Zhangshu 331200, China
4. The Affiliated Hospital of Jiangxi University of TCM, Nanchang 330006, China
- Publication Type:Research Article
- Keywords:
Cinnamomi Ramulus;
75% alcohol extract;
chemical constituents;
structural identification
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):173-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The research group found in the early stage that the 75%alcohol extract of the Cinnamomi Ramulus had a significant physiological activity in inhibiting necroptosis by screening out the self-built sample library of 100 kinds of traditional Chinese medicines in Jiangxi. To identify the active components and find the target compounds,the 75%alcohol extracts of Cinnamomi Ramulus were isolated and studied systemically in chemistry. Method:The 20 kg dry Cinnamomi Ramulus was crushed into coarse powder,and extracted with 75%alcohol for four times, one time every 7 d. Then total extracts were obtained after solvent was recycled under decompression. The extract was separated by D101 macroporous resin column chromatography and eluted by water,30%ethanol,50%ethanol,70%ethanol,90%ethanol,so as to get the corresponding fraction finally. The compounds in the 30%ethanol and 50%ethanol fraction were isolated and purified by chromatography on silica gel,Sephadex LH-20 column and high pressure preparative chromatography,and their structures were determined according to physicochemical properties and spectral analysis. Result and Conclusion:Thirteen compounds were isolated and identified as (+)-syringaresinol (1),(+)-lyoniresinol (2),spicatolignan B (3),(-)-secoisolariciresinol (4),ovafolinin B (5),protocatechualdehyde (6),protocatechuic acid (7),syringaldehyde (8),vanillic acid (9),ethyl protocatechuate (10),syringic acid (11),ethyl gallate (12),2-(3',4'-dihydroxyphenyl)-1,3-pepper ring-5-aldehyde (13). Compounds 1-5,10-13 were isolated from this plant for the first time.
