Investigation of Skin Irritation of Essential Oils from Interior-warming Medicines
10.13422/j.cnki.syfjx.20190548
- VernacularTitle: 温里药挥发油的皮肤刺激性考察
- Author:
Yun-fei DONG
1
;
Feng-ye WU
1
;
Jiang-an XI
1
;
Han FENG
1
;
Yong-kun WEN
1
;
Jun-hong YAO
1
;
Xue-min ZHU
1
;
Jie DONG
1
;
Ye-qing QU
1
;
Jun CHEN
1
Author Information
1. School of Pharmacy, The First Clinical Medical College, School of Health Economics and Management, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Drum Tower Clinical College of Integrated Traditional Chinese Medicine and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
- Publication Type:Research Article
- Keywords:
interior-warming medicines;
essential oils;
skin cytotoxicity;
in vivo skin irritation;
skin test panels;
half-inhibitory concentration;
rhodamine B
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):156-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the skin irritation of essential oils(EOs) extracted from interior-warming medicines. Method:Three EOs from interior-warming medicines(Cinnamomi Cortex, Caryophylli Flos and Alpiniae Officinarum Rhizoma) were selected as research objects.The in vitro skin cytotoxicity and in vivo skin irritation of these EOs were determined and compared.Moreover, the skin irritation was also predicted by the novel skin test panels. Result:Toxicity of these three EOs to human skin fibroblasts(HSF) was significantly different, half-inhibitory concentration(IC50) values of EOs from Cinnamomi Cortex, Alpiniae Officinarum Rhizoma and Caryophylli Flos were (11.16±0.28), (53.33±1.71), (226.70±17.61) mg·L-1, respectively.However, in vivo skin irritation evaluation showed that the local toxicity of these three EOs was in the order of EO of Cinnamomi Cortex > EO of Caryophylli Flos > EO of Alpiniae Officinarum Rhizoma. The evaluation results of skin test panels for these three EOs were in accordance with the results of in vivo skin irritation evaluation. Conclusion:Toxicity of these three EOs against skin cells in vitro is inconsistent with their in vivo skin irritation. Skin test panels are expected to be able to accurately predict in vivo skin irritation of EOs instead of cytotoxicity evaluation.
