Comparation of Effect on Tripterygium Glycosides Tablet from Two Different Manufacturers in CIA Rats
10.13422/j.cnki.syfjx.20191437
- VernacularTitle: 两个厂家的雷公藤多苷片对CIA模型大鼠干预作用比较
- Author:
Li-ling LIU
1
;
Xiao-hui SU
2
;
Ya-ge TIAN
2
;
Yuan-fang FAN
2
;
Hong-feng WANG
3
;
Wei-hong PAN
4
;
Xue-ying WANG
4
;
Hua-jun PENG
3
;
Ying XU
2
;
Xiang-ying KONG
2
;
Wei CAO
1
;
Na LIN
2
Author Information
1. Guanganmen Hospital, China Academy of Chinese Medicine Sciences, Beijing 100053, China
2. Institute of Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences, Beijing 100700, China
3. Hunan Qianjin Xieli Pharmaceutical Limited Company, Zhuzhou 412000, China
4. Zhejiang Deende Pharmaceutical Limited Company, Shaoxing 312000, China
- Publication Type:Research Article
- Keywords:
Hunan Qianjin Xieli tripterygium glycosides tablet;
Zhejiang Deende tripterygium glycosides tablet;
rheumatoid arthritis;
collagen-induced arthritis rat model;
anti-arthritis effect;
toxicity
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):84-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the effects and multi-organ intervention of tripterygium glycosides(TG) tablet from Hunan Qianjin Xieli (QJ) and Zhejiang Deende (DED) on type Ⅱ collagen-induced arthritis (CIA) in rats. Method:The 72 SD rats were randomly divided into normal group, model group, QJ TG clinical group 2 times, 6 times equivalent dose group (QJ-TG 0.018, 0.054 g·kg-1), derende TG clinical group 2 times, 6 times equivalent dose group (DED-TG 0.018, 0.054 g·kg-1). The intragastric administration was started on the day after the first immunization, once a day. After the second immunization, the symptoms such as redness and swelling of joints were observed, and the clinical score of arthritis were evaluated. The materials were taken for pathological examination of the inflammatory joints on the 21th and 42th day. The concentration of alkaline phosphatase(ALP), alanine aminotransferase(ALT), aspartate aminotransferase(AST), gamma-glutamyltransferase(GGT), total bilirubin(TBIL), creatinine(CRE) and urea(UREA) in serum were detected by enzymatic assay. The rate of sperm deformity, testicular and ovarian tissue damage in the rat epididymis was assessed. Result:TG from two manufacturers attenuated the inflammation, redness, swelling and deformity of joints in CIA rats, reduced the clinical score and incidence of arthritis in CIA rats. Meanwhile, it also exhibited obvious reduction in all pathological features such as joint synovitis, pannus, cartilage erosion and bone destruction. There were significant differences between the QJ-TG high and low dose groups and the DED-TG high dose group compared with the model group (P<0.05, P<0.01). There was no significant change in the low dose group of DED-TG compared with the model group.Compared with the same dose of TG in the two manufacturers, the DED-TG 0.054 g·kg-1 group had a significant inhibitory effect on the clinical scores on the 15th and 18th days than the QJ-TG same dose group (P<0.05).In addition to 0.054 g·kg-1 dose of DED-TG, the white blood cell count and spleen index were significantly increased.At the same time, two different manufacturers of TG had no effect on body weight, organ index, digestive system, liver and kidney function, liver and kidney pathology of CIA model rats. QJ-TG and DED-TG all significantly increased the rate of male rats sperm malformation and significant damage to testicular seminiferous tubules and the toxicity increased with the increase of dose and time. while the mole reproductive toxicity of DED-TG was higher than that of QJ-TG at the same dose. In the DED-TG 0.054 g·kg-1 and QJ-TG 0.054 g·kg-1 group, there were only the reduction of vascular distribution in the ovarian tissue and the reduction of the corpus luteum, and no other toxic effects were observed. Conclusion:Two manufacturers TG2 times (0.018 g·kg-1) and 6 times (0.054 g·kg-1) clinical equivalent dose can delay the onset of CIA in rats, reduce the clinical score of arthritis, improve the pathological changes of joints, but have a certain degree of male reproductive toxicity. The high-dose DED-TG is more toxic than the QJ-TG.
