Effect of Shuangyu Tiaozhi Decoction on NPC1L1 and ABCG8 in Intestine of Hyperlipemic Model Rats
10.13422/j.cnki.syfjx.20191402
- VernacularTitle: 双蓣调脂汤对高脂血症模型大鼠小肠组织NPC1L1及ABCG8表达的影响
- Author:
Ruo-qi LI
1
;
Jing-jing SHI
1
;
Hai-fei LU
1
;
Lu YU
1
;
Feng-xia ZHANG
2
Author Information
1. Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China
2. Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Ji'nan 250011, China
- Publication Type:Research Article
- Keywords:
Shuangyu Tiaozhi decoction;
spleen tonifying turbid method;
hyperlipidemia;
niemann-pick C1-like 1 (NPC1L1);
liver X receptor-α (LXR-α);
adenosine triphosphate-binding cassette transporters G8 (ABCG8)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):77-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression levels of niemann-pick C1-like 1 (NPC1L1) and adenosine triphosphate-binding cassette transporters G8 (ABCG8) in intestine of hyperlipidemic model rats, in order to investigate the therapeutic mechanism of Shuangyu Tiaozhi decoction on hyperlipidemia. Method:A total of 40 SD rats were selected, including 8 for normal control group. The remaining 32 rats were used to establish hyperlipemic model. After modeling, the rats were randomly divided into the model group (equivalent normal saline), the high and low-dose Shuangyu Tiaozhi groups (15.6, 7.8 g·kg-1), and the Simvastatin group (4 mg·kg-1), with 8 in each group. They were given drugs by gavage for 8 weeks. The levels of total cholesterol (TC), triglyceride (TG) in serum and total cholesterol (TTC), free cholesterol (FTC) in liver of rats in each group were determined by biochemical and enzymatic methods. The morphological changes of liver were observed by hematoxylin-eosin (HE) staining, and the levels of expressions of NPC1L1, ABCG8 and liver X receptor-α (LXR-α) in intestine were detected by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) and Western blot. The expression of ABCG8 protein was determined by immunohistochemistry. Result:After successful replication of the hyperlipidemia model, the blood lipid level was abnormally increased, and the liver steatosis became obvious in the model group compared with the normal control group. The expression levels of NPC1L1, LXR-α and ABCG8 increased significantly (P<0.05, P<0.01). Compared with the model group, the blood lipid levels were significantly reduced in the Shuangyu Tiaozhi decoction group. The expressions of NPC1L1 and LXR-α were significantly down-regulated, but ABCG8 was obviously up-regulated in a dose-dependent manner (P<0.05, P<0.01). The results of immunohistochemistry indicated that the protein expression of ABCG8 in intestine of the model group increased significantly (P<0.01). Compared with the model group, the expression of ABCG8 in each drug-administered groups increased obviously (P<0.05, P<0.01), especially in high-dose Shuangyu Tiaozhi group (P<0.05). Conclusion:Shuangyu Tiaozhi decoction can reduce the blood lipid level of hyperlipemic rat model by reducing the absorption of cholesterol. Its mechanism may be correlated with the down-regulation of NPC1L1 expression and the up-regulation of ABCG8 expression.
