Neuroprotective Mechanism of Buyang Huanwu Tang on Experimental Autoimmune Encephalomyelitis Mice
10.13422/j.cnki.syfjx.20191340
- VernacularTitle: 补阳还五汤对自身免疫性脑脊髓炎模型小鼠神经保护作用的机制探讨
- Author:
Jian-chun LIU
1
;
Hong-zhen ZHANG
1
;
Wen-juan GUO
1
;
Zhi CHAI
1
;
Jie-zhong YU
2
;
Jing-wen YU
2
;
Bao-guo XIAO
3
;
Cun-gen MA
1
Author Information
1. Research Center of Neurobiology, Shanxi University of Chinese Medicine, Jinzhong 030619, China
2. Institute of Brain Science, Datong University, Datong 037009, China
3. Institute of Neurology, Huashan Hospital, Fudan University, Shanghai 200025, China
- Publication Type:Research Article
- Keywords:
Buyang Huanwu Tang;
experimental autoimmune encephalomyelitis;
brain-derived neurotrophic factor;
growth-related protein-43;
Nogo-A protein;
nerve growth microenvironment
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):55-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the neuroprotective effect and mechanism of Buyang Huanwu Tang (BYHWT) on experimental autoimmune encephalomyelitis (EAE) at different stages. Method:The 36 female C57BL/6 mice were immunized subcutaneously with myelin oligodendrocyte glycoprotein peptides (MOG35-55),then randomly divided into 9, 17, 28 d EAE control group. Each BYHWT group was orally given drugs on the 3rd day after immunization (50 g·kg-1·d-1), and EAE control group was given the same volume of normal saline in the same way once a day for 9, 17 and 28 d after immunization. The effect of BYHWT on EAE mice was observed with internationally accepted clinical score. Brain and spinal cord specimens were collected at 9, 17 and 28 d after immunization. The neuroprotective effect of BYHWT was observed by hematoxylin-eosin(HE)staining and solid blue staining (LFB). The expressions of BDNF and GAP-43 in spinal cord and brain were detected by Western blot. Result:After treatment, BYHWT can significantly inhibit myelitis cell infiltration and alleviate myelin loss. Compared with EAE group, the expression of Nogo-A in the spinal cord of each BYHWT group was significantly down-regulated (P<0.01), and the expression of BDNF in the spinal cord was significantly up-regulated (P<0.05, P<0.01) in the BYHWT group 17 and 28 d group compared with EAE group 17 and 28 d group. Compared with EAE 9, 17 d group, GAP-43 expression was significantly up-regulated in the spinal cord of BYHWT 9, 17 d group (P<0.01). Conclusion:BYHWT can improve the local nerve growth microenvironment and promote the expression of NTFs, reduce the expressions of neuroinhibitory factors, and play a role in neuroprotection.
