Effect of Aloesin on Proliferation and Apoptosis of Non-small Cell Lung Cancer A549 Cells
10.13422/j.cnki.syfjx.20191323
- VernacularTitle: 芦荟苦素对非小细胞肺癌A549细胞增殖及凋亡的影响
- Author:
Yang-zhuang-zhuang ZHU
1
;
Fei ZHANG
2
;
Xiao-ning JIAO
1
;
Lin SU
1
;
Lu-yao WEI
1
;
Xian-dan ZHU
1
;
Zhen-zhen HUANG
1
;
Shi-guo ZHU
1
;
Xiao CHEN
1
;
Chun-pu ZOU
1
;
Zi-hang XU
1
Author Information
1. School of Basic Medical Science, Application and Research Center of Classical Prescription, Science and Technology Experiment Center, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
2. Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
- Publication Type:Research Article
- Keywords:
aloesin;
non-small cell lung cancer;
inhibition of cell proliferation;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):13-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of aloesin in inducing apoptosis in human non-small cell lung cancer (NSCLC) A549 cells, so as to inhibit its proliferation. Method:A549 cells in logarithmic growth phase were collected, and cell counting kit-8 (CCK-8) was used to detect the effect of different concentrations of aloesin (2, 4, 8, 16, 32, 64, 128 μmol·L-1) on the proliferation of A549. Effect of aloesin (0, 16 μmol·L-1) on the number of clones formed in A549 cells and the size of clone formation was determined by crystal violet staining. effect of aloesin on apoptosis of A549 cells was detected by annexin V/propidium iodide(PI)apoptosis kit staining. Hoechst staining was used to detect the phenomenon of apoptotic nuclear pyknosis. Western blot was used to detect aloesin's effect on death-related protein expressions of Bcl-xl/Bcl-2 associated death promoter (Bad), cleaved-Caspase-3,cl-Caspase-3(Asp175), Caspase-3, cleaved poly ADP-ribose polymerase (cl-PARP), poly ADP-ribose polymerase (PARP) in A549 cells. In vivo, 5-week-old nude mice were subcutaneously inoculated with 2×106 A549 cells, and randomly divided into the medication group and the blank group. aloesin or normal saline was intraperitoneally injected for 4 weeks, and the tumor volume of nude mice was measured weekly. The body weight of the mice was observed, and the appearance of the nude mice was observed. Result:Aloesin inhibited the proliferation and cloning of A549 cells in a concentration-dependent manner (P<0.05). After treatment with aloesin, the number of apoptosis and the phenomenon of nuclear pyknosis in A549 cells increased significantly (P<0.01). At the same time, aloesin significantly down-regulated the expression of apoptosis-related protein Bcl-xl (P<0.05), and increased the expression of Bad protein (P<0.01). The expression levels of cl-PARP (P<0.01) and cl-Caspase-3 (P<0.05) were also significantly increased. In addition, in vivo, aloesin significantly shrank the volume of subcutaneous tumors in mice, reduced tumor weight, with a better appearance than that of the control group. Conclusion:Aloesin may inhibit the expression of NSCLC by inducing apoptosis of A549 cells, and is safe to use, with no inhibitory effect on the body weight of mice.
