Inhibitory Effect of Zeqi Tang on Mouse Model of Lung Cancer
10.13422/j.cnki.syfjx.20191322
- VernacularTitle: 泽漆汤对肺癌原位模型小鼠的抑制作用
- Author:
Zi-hang XU
1
;
Yang-zhuang-zhuang ZHU
1
;
Fei ZHANG
2
;
Lu-yao WEI
1
;
Lin SU
1
;
Xiao-ning JIAO
1
;
Qin ZHOU
1
;
Ning ZHANG
1
;
Zhen-zhen HUANG
1
;
Xian-dan ZHU
1
;
Fei LIU
1
;
Hai-rong ZHONG
1
;
Shi-guo ZHU
1
;
Xiao CHEN
1
;
Chun-pu ZOU
1
Author Information
1. Application and Research Center of Classical Prescription, School of Basic Medical Science, Center for Traditional Chinese Medicine(TCM) and Systems Biology, Science and Technology Experiment Center, Shanghai University of TCM, Shanghai 201203, China
2. Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai 200092, China
- Publication Type:Research Article
- Keywords:
Zeqi Tang;
pulmonary mouse model;
NK cells;
quality evaluation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(14):6-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe effect of Zeqi Tang in intervening mice with orthotopic lung cancer model, in order to observe its anti-tumor mechanism. Method:An in situ mouse model of non-small cell lung cancer was established through intrapulmonary injection with 1×105 LLC-luc cells. The model mice were intragastrically administered with Zeqi Tang(0.171 g·mL-1) or normal saline for 35 days. Appearance (spirit, hair, appetite, sleep), survival period and Zeqi Tang anti-tumor effect were observed, weekly vital imaging was performed to detect the fluorescence signal in the lungs of mice. Flow cytometry was used to detect the NK cell content in the spleen of the model mice. CD107α was used to detect the degranulation of NK cells in the spleen of mice after administration of Zeqi Tang. Kromasil 100 5 C18 column was used and eluted with acetonitrile-0.025%phosphoric acid in a gradient mode, with flow rate at 1.0 mL·min-1, column temperature at 35℃ and detection wavelength of 265 nm, as to establish the fingerprint of Zeqi Tang. The fingerprints of 10 batches of samples was evaluated by using the Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System Software (2012 Edition) recommended by the Chinese Pharmacopoeia Commission, in order to complete the quality control of Zeqi Tang. Result:Zeqi Tang could significantly inhibit the lung fluorescence signal of lung cancer in situ model mice and prolong the survival of mice(P<0.05, P<0.01). After the intervention with Zeqi Tang, the NK cells in the tumors increased significantly(P<0.01), and the degranulation of CD107α also increased significantly(P<0.01). Moreover, the HPLC-DAD fingerprint of Zeqi Tang showed a significant increase in the fingerprint similarity of 10 batches of lacquer soup aqueous extract. Moreover, the HPLC-DAD fingerprint of Zeqi Tang showed that the fingerprint similarity of 10 batches of lacquer soup aqueous extract was ≥ 0.9, indicating that small differences between the batches. Conclusion:Zeqi Tang may enhance the tumor growth and prolong the survival period of mice by up-regulating the number of NK cells in mice and enhancing their degranulation function. The evaluation of similarity of HPLC fingerprint of Zeqi Tang reflects the quality of lacquer soup to a certain extent, and can provide reference for further study.
