Quantitative and Fingerprint of Ilex pubescens Slices
10.13422/j.cnki.syfjx.20190818
- VernacularTitle: 毛冬青饮片指纹图谱及定量分析
- Author:
Hong-xing MA
1
;
Long-fei LIN
2
;
Yu-ling LIU
2
;
Sai FU
2
;
Jin-xin SHAO
3
;
Ji-zhong ZHU
4
;
Chuan-gui LIU
4
;
Hui LI
2
;
Wen-cong LIU
1
Author Information
1. Jilin Agricultural University, Changchun 130118, China
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
3. Chengde Medical University, Chengde 067000, China
4. Jilin Huakang Pharmaceutical Co. Ltd., Dunhua 133700, China
- Publication Type:Research Article
- Keywords:
Ilex pubescens;
root;
stem;
fingerprints;
HPLC;
Ilex pubescens saponins A1;
Ilex pubescens saponins B1
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):140-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish HPLC-UV fingerprints of Ilex pubescens pieces,and simultaneously determine two components in 46 batches of I. pubescens in pieces of I. pubescens saponin A1 and B1,in order to provide a reference for the quality standard of I. pubescens slices. Method:Methanol was used to extract the I. pubescens saponin samples,and the extracts were measured by HPLC-UV with the absorption wavelength at 210 nm. Kromasil C18 column (4.6 mm×250 mm,5 μm) was used for determining the extracts at a flow rate of 1.0 mL·min-1. The mobile phase condition was acetonitrile-0.1% phosphoric acid aqueous solution with gradient mode. The chromatographic fingerprint similarity evaluation system of traditional Chinese medicine (2012 edition) was used to analyze I. pubescens fingerprints. SPSS 20.0 software was used to cluster the peak area of common peaks. Principal component analysis was performed to reduce the dimension of common peaks. Result:There were great differences between the root and stem parts in I. pubescens fingerprints. The fingerprints of roots and stems of I. pubescens were established respectively,cluster results assorted the roots of I. pubescens into three categories andthe branches of I. pubescens into two categories. The integrity and difference of I. pubescens decoction pieces from different parts and places of origin were compared,and the principal component analysis was performed to screen out the common components that played a decisive role in fingerprint of I. pubescens pieces. And the common peaks were determined. The content of saponin A1 and saponin B1 in Radix I. pubescens were determined. Conclusion:The established I. pubescens fingerprints and content determination methods are simple and suitable. Cluster analysis and principal component analysis are used to screen out the key components of quality control of I. pubescens. The results can provide references for quality control of I. pubescens.
