Protective Effect of Ferulic Acid on PC12 Cells with H2O2-induced Oxidative Damage
10.13422/j.cnki.syfjx.20191202
- VernacularTitle: 阿魏酸对H2O2致PC12细胞氧化损伤的保护作用
- Author:
Qiu-shan LIN
1
;
Pei-fen YANG
1
;
Man-xue YIN
1
;
Shi-bing ZHANG
1
;
Si-jun LIU
1
;
Qing-guang WU
1
Author Information
1. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
- Publication Type:Research Article
- Keywords:
ferulic acid;
PC12 cells;
oxidative stress;
protective effect
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):66-72
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of ferulic acid on PC12 cells injured by H2O2 and the molecular mechanisms. Method:The oxidative stress model was established by treating PC12 cells with H2O2, and then different dosages of ferulic acid (1, 10, 100 μmol·L-1) were used for intervention. Methyl thiazolyl tetrazolium (MTT) assay was used to evaluate the cell viability,lactate dehydrogenase (LDH) and malondialdelyde (MDA) in cell supernatant, and superoxidedismutase (SOD) in cells was tested by biochemical method respectively. Insulin-like growth factors-1 (IGF-1) mRNA and protein expressions were detected by Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR) and Western blot. Result:The 24 h intervention with different dosages of ferulic acid (1, 10, 100 μmol·L-1) could significantly improve the oxidative damage of PC12 cells induced by H2O2, compared with the model group, ferulic acid at 1,10,100 μmol·L-1 significantly increased PC12 cells viability,significantly decreased LDH and MDA content in cell supernatant (P<0.05, P<0.01), and enhanced SOD activity(P<0.01). Real-time PCR and Western blot showed that compared with control group,IGF-1 mRNA and protein expressions in model group decreased significantly (P<0.01), compared with model group, IGF-1 mRNA and protein expressions in ferulic acid group increased significantly (P<0.01). Conclusion:Ferulic acid exerts a protective effect on H2O2-inducing PC12 cells injury,which might be related to insulin signaling pathways.
