Effect of Pudilan Xiaoyan Oral Liquid on Acute Lung Injury Rat Induced by Lipopolysaccharide
10.13422/j.cnki.syfjx.20191338
- VernacularTitle: 蒲地蓝消炎口服液对脂多糖致大鼠急性肺损伤的影响
- Author:
Yi-fei YANG
1
;
Guang-ping ZHANG
1
;
Yun-hang GAO
1
;
Meng MA
1
;
Shuang-rong GAO
1
;
Han LI
1
;
Ling SONG
1
;
Zu-guang YE
1
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
Pudilan Xiaoyan oral liquid;
acute lung injury;
lipopolysaccharide;
inflammatory factor;
immune regulation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):55-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of Pudilan Xiaoyan oral liquid(PDL) on the acute lung injury rat induced by lipopolysaccharide (LPS). Method:The 72 Wistar rats were randomly divided into control group, model group, dexamethasone group, PDL 7, 3.5, 1.75 g·kg-1·d-1 group according to body weight.The acute lung injury model was made through inhalation with lipopolysaccharide in the model group, hexadecadrol group, PDL 7, 3.5, 1.75 g·kg-1·d-1 group.To examining each rat alveolar lavage fluid (BALF) of the total number of white blood cells, enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of nuclear transcription factors-kappa B (NF-κB) and interleukin-10 (IL-10).Hematoxylin-eosin(HE) staining was used to observe morphological changes of lung tissue and explore different doses of PDL effect on acute lung injury in rats. Result:Compared with model group, the account of leukocyte in BALF decreased significantly in PDL 7 g·kg-1·d-1 group and PDL 3.5 g·kg-1·d-1 group (P<0.05). The expression of NF-κB significantly decreased in PDL 7, 3.5, 1.75 g·kg-1·d-1 group (P<0.05). And the expression of IL-10 significantly increased in PDL 3.5 g·kg-1·d-1 group (P<0.05). In PDL 7 g·kg-1·d-1 group, the inflammation, edema and congestion in lung tissue reduced (P<0.05). Conclusion:PDL has a significant protective effect on the inflammation of acute lung injury model, and its mechanism is related to the expressions of NF-κB and IL-10. PDL could also repair the injury of lung in acute lung injury model.
