Evaluation of Compatibility Proportion and Antidepressant Mechanism of Bupleuri Radix-Scutellariae Radix Based on Uniform Design
10.13422/j.cnki.syfjx.20190451
- VernacularTitle: 基于均匀设计法评价柴胡-黄芩的配伍比例及抗抑郁机制
- Author:
Li-ping ZHOU
1
;
Guo-chun LI
1
;
Chen-yi-yu ZHANG
1
;
Fan ZHAO
1
;
Ya-qin LI
1
;
Shi-ping MA
2
;
Rong QU
1
Author Information
1. School of Medicine and Life Sciences, School of Basic Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, China
2. School of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210038, China
- Publication Type:Research Article
- Keywords:
depression;
Bupleuri Radix-Scutellariae Radix;
compatibility proportion;
uniform design;
cyclic adenosine monophosphate-response element binding protein;
brain-derived neurotrophic factor;
behavior tests
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):28-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize proportion of couplet medicine of Bupleuri Radix and Scutellariae Radix in the treatment of anti-depression, and to explore the possible antidepressant mechanism of this couplet medicine. Method:The dosages of Bupleuri Radix and Scutellariae Radix in the 2015 edition of Chinese Pharmacopoeia were taken.According to U7(72) uniform design table, Bupleuri Radix and Scutellariae Radix were carried out into 7 groups.The chronic unpredictable mild depression model mice were induced by intragastric administration of decoction of this couplet medicine and the antidepressant effect was observed by the behavior tests, which included sucrose preference test, tail suspension test, forced swimming test and open field test(crossing scores).The regression equations were established by selecting the effective indexes.The experiments of retest were taken to check the results and the possible antidepressant mechanism was primarily investigated by measuring the phosphorylation level of cyclic adenosine monophosphate(cAMP)-response element binding protein(CREB) and expression of brain-derived neurotrophic factor(BDNF). Result:Compared with the blank group, sucrose preference rate of the model group was significantly decreased(P<0.01), the number of crossing was also obtained a significant decrease(P<0.01) in the model group, the immobility times of forced swimming and tail suspension were prolonged obviously(P<0.01) in the model group.The decoction of Bupleuri Radix-Scutellariae Radix could reverse the above indexes of model mice, the sucrose preference rate and the number of crossing were increased(P<0.05, P<0.01), the immobility times of forced swimming was evidently decreased(P<0.01) and the immobility time of tail suspension was also decreased(P<0.05, P<0.01).The experiment of retest showed that the decoction of Bupleuri Radix-Scutellariae Radix could improve depressive behavior of model mice, sucrose preference rates were significantly increased(P<0.01), the immobility time of forced swimming was significantly shorted(P<0.01) and the time of tail suspension was also decreased(P<0.05, P<0.01).The Bupleuri Radix-Scutellariae Radix(1:1) and (8:3) groups could increase the number of crossing (P<0.05, P<0.01) of model mice, what's more, the Bupleuri Radix-Scutellariae Radix(1:1) group was the most effective than the other groups and it could up-regulate phosphorylation levels of CREB and the expression of BDNF in the hippocampus. Conclusion:Compatibility of Bupleuri Radix and Scutellariae Radix can ameliorate depressive-like behavior of model mice, and the best antidepressant compatibility proportion of Bupleuri Radix and Scutellariae Radix is 1:1, the optimal amounts of them are about 5 g.The antidepressant effect may relate to promoting phosphorylation level of CREB and the expression of BDNF in the hippocampus.
