Effect of Modified Shaoyao Gancao Tang on MPTP-induced Parkinson's Disease Model Mice
10.13422/j.cnki.syfjx.20191141
- VernacularTitle: 芍药甘草汤加减对MPTP诱导的帕金森病模型小鼠的影响
- Author:
Shao-chen QIN
1
;
Ai-mei WANG
1
;
Ruo-yu LI
1
Author Information
1. The Hospital of Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China
- Publication Type:Research Article
- Keywords:
modified Shaoyao Gancao Tang;
Parkinson's disease;
apoptosis;
oxidative;
neuroprotective
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(13):15-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of modified Shaoyao Gancao Tang on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease model mice. Method:The sixty C57/BL6 mice were randomly divided into normal group, model group, Madopar group (50 mg·kg-1) and low, medium and high doses modified Shaoyao Gancao Tang (1,2,4 g·kg-1). The modeling method was to intraperitoneally inject C57/BL6 mice with MPTP(40 mg·kg-1) once a day for 7 days. Except normal group and model group were given normal saline daily,drug-administered group was intragastrical administered once a day, and the third day after the drug was administered according to the above method (except normal group). The behavior of each group of mice (climbing test, swimming test, and tail suspension test) was examined up to 15 days after administration. Subsequently, the levels of Cystatin-C (Cys-C) and neuron-specific enolase (NSE) in the serum of each group were detected by enzyme-linked immunosorbent assay (ELISA). The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in striatum of each group were detected by spectrophotometry. The protein expression of α-synuclein (α-syn), tyrosine hydroxylase (TH),B lymphoma-2 gene (Bcl-2) and Bcl-2 related X protein (Bax) in the striatum of each group was detected by Western blot. Result:Compared with normal group, the swimming experiment scores and suspension experiments scores in the behavioral experiments of model group mice were significantly lower (P<0.01),The climbing time was significantly increased (P<0.01); the serum Cys-C content was significantly decreased (P<0.01),serum NSE content increased significantly (P<0.05). SOD level and GSH-Px level in striatum decreased significantly (P<0.01),MDA level in striatum increased significantly (P<0.05), TH and Bcl-2 expression in striatum were significantly decreased (P<0.01),α-syn and Bax expression in striatum were significantly increased (P<0.05). Compared with model group, the scores of swimming experiment and suspension experiment in the behavioral experiments of Shaoyao Gancao Tang group and Madopar group were significantly increased (P<0.05),while the climbing time was significantly reduced (P<0.05). The serum Cys-C content increased significantly (P<0.05),the serum NSE content decreased significantly (P<0.05). The SOD level and GSH-Px level in the striatum increased significantly (P<0.05),while the MDA level in the striatum decreased significantly (P<0.05). There was also a significant increase in TH and Bcl-2 expression in the striatum (P<0.05),α-syn and Bax expression in striatum were significantly decreased (P<0.05). Compared with Madopar group,the scores of swimming experiment and suspension experiment in the behavioral experiment of high-dose group of Shaoyao Gancao Tang were significantly increased (P<0.05),while the climbing time was significantly reduced (P<0.05). The serum Cys-C content increased significantly (P<0.05),while the serum NSE content decreased significantly (P<0.05).The SOD level and GSH-Px level in the striatum increased significantly (P<0.05),while the MDA level in the striatum decreased significantly (P<0.05). There was also a significant increase in TH expression and Bcl-2 expression in the striatum (P<0.05),while α-syn expression and Bax expression in the striatum were significantly decreased (P<0.05). Conclusion:The neuroprotective effect of modified Shaoyao Gancao Tang on MPTP-induced Parkinson's disease model mice may be achieved by inhibiting oxidative damage and apoptosis.
